Microbial communities of uranium contaminated groundwater : Metagenomic insights

1. Microbial communities of uranium contaminated groundwater : Metagenomic insights Group members: Pinaki Sar Ratul Saha Sufia Kazy

2. Microbial communities from highly uranium-contaminated groundwater were sequenced using random shotgun sequencing-based strategy with the aim of addressing the following questions: • How does anthropogenic environmental change affect groundwater microbial community diversity and structure? • How does microbial community adapt to severe environmental changes such as heavy metal contamination? • Can molecular mechanisms responsible for such environmental changes be predicted from the metagenomic sequence?

3. Our questions • What are the dominant populations in the two metagenomes obtained from ORFRC • What are the functional differences between metagenomes? • How the phylogenetically abundant members are functionally relevant?

4. Samplings location (ORFRC) uranium, technetium-99 , nitrate, thorium, and volatile organic compounds such as acetone, methylene chloride, toluene, and tetrachloroethylene

5. Metagenomic statistics

6. Phylogenetic distribution

7. Phylogenetic distribution of Xanthomonadaceae members

9. COGs from contaminated and pristine samples related to X. campestris Contaminated sample Pristine sample

10. Distribution of protein coding genes with COGs within the contaminated samples

11. Distribution of protein coding genes with COGs within the pristine samples

12. Protein coding genes detected within the contaminated samples connected to KEGG pathways

13. Protein coding genes detected within the pristine samples connected to KEGG pathways

14. Abundance Profile

15. Phylum

16. Family Composition

17. Species Composition

18. …..and more questions……….. Conclusion