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a. b. c. #55. #99. d. e. f. #221. #238. Glut. SO 4 2-. SO 4 2-. Glut. Glut. b. e. a. d. f. c. #162. #270. 31nM Ca 2+ in Glut; 174nM Ca 2+ in SO 4 2-. 012204a. 012204a. SO 4 2-. EGTA. 110904d. 50 μ M TBQ. SO 4 2- , 100 nM Ca 2+ , 0.4 mM Mg 2+.

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Glut

a

b

c

#55

#99

d

e

f

#221

#238

Glut

SO42-

SO42-

Glut

Glut

b

e

a

d

f

c

#162

#270

31nM Ca2+ in Glut; 174nM Ca2+ in SO42-

012204a


Glut

012204a


Glut

SO42-

EGTA

110904d

50 μM TBQ

SO42-, 100 nM Ca2+, 0.4 mM Mg2+

50 mM EGTA, 1 mM Mg2+

111804b


Glut

Glut

SO42-

Glut

033004a


Glut

Table 1: Sparks parameters from expts on Leica.


Glut

A

0 s

2 min 5 s

3 min 5 s

4 min 30 s

11 min 30 s

B

15 min 22 s

17 min 22 s

22 min 22 s

12 min 53 s

13 min 56 s

C

23 min 53 s

24 min 54 s

26 min 23 s

28 min 23 s

D

Glut

Glut

50 Pi

Cell region

Whole

SR [Ca2+] (μM)

Edge

Middle

Fig x: The effect of inorganic phosphate on SR [Ca2+]. Organelle-trapped mag-indo showing the change in SR [Ca2+] in the presence of cytoplasmic solutions containing K-glutamate, 1 mM EGTA (200 nM Ca2+) and 0.4 mM Mg2+ (A), 50 mM Pi, 1 mM EGTA (200 nM Ca2+) and 1 mM Mg2+(B) and then upon return to K-glutamate (C). The time passed since the start of the experiment is noted on each panel. The image at time 0 was in the presence of 0 Ca, 0 ATP (“relaxing”) solution. The SR [Ca2+] changes are summarized in D. Note that there were significant spatial SR [Ca2+] gradients between the edge and middle of the cell in C (indicated by the blue and green bars, respectively), which are plotted in D. The whole cell SR [Ca2+] average is plotted in red in D. Fiber identifier: 011604a.


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