1 / 7

Self-sufficiency in growth signals

Self-sufficiency in growth signals. Sustained angiogenesis. Insensitivity to anti-growth signals. Evading apoptosis. Tissue invasion & metastasis. Limitless replicative potential. Cancer. The development of human cancer cells.

alta
Download Presentation

Self-sufficiency in growth signals

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. Self-sufficiency in growth signals Sustained angiogenesis Insensitivity to anti-growth signals Evading apoptosis Tissue invasion & metastasis Limitless replicative potential Cancer The development of human cancer cells

  2. Cdc6 is essential in the initiation of DNA replication: A). Assembling pre-replication complex (pre-RC) with ORC, and loading MCMs to replication origins; B). Licensing DNA replication to ensure one round DNA replication per cell cycle.

  3. LA-N-2 MCF7 CHLA255 Cdc6 shRNA THTD6Ai THTD6Ai THTD6Ai THTP Mock Mock THTP THTP Cdc6 Cdc6 knockdown -actin 1 2 3 4 5 6 7 8 The novel function of GLS101/TSUKUSI FL1 is the silencing of human Cdc6, the specific genes for cell proliferation: Specific Cdc6 shRNAs produced from the viral vector Inhibit Cdc6 and block Cancer cell proliferation, but not normal cells.

  4. Apoptosis Differentiation M G1 Recombinant Lentivirus GLS101 G2 STOP S Senescence The Cell Cycle

  5. ——   THTD  ———IP/mouse IgG —    IP/β-p16INK4a Phosph-Rb-C Ser780 Phosph-Rb-C Ser807/811 IgG, light chain 1 2 3 4 ——— THTD 1:10 1:25 1:5  IP/β-p16INK4a Phosph Rb-C Ser 807/811 IgG, light chain 1 2 3 4 Reactivation of p16INK4a CDK inhibitory activity by Cdc6 knockdown

  6. MCF7 MCF7/THTP MCF7/THTD6Ai Senescence-associated β-glactosidase (SA-β-Gla) positive staining caused by Cdc6 knockdown

  7. Anti-H3K9me3/me2 indirect fluorescent staining Mock THTD6Ai Confocal fluorescent microscopy Mock THTD6Ai The formation of senescence associated heterochromatic foci via Cdc6 knockdown

More Related