In the name of god
Download
1 / 28

In the name of God - PowerPoint PPT Presentation


  • 325 Views
  • Updated On :

In the name of God. Diagnostic microbiology. Background. Microbiology is the study of cellular and acellular cells and agents. Cellular micro-organisms are consisted of prokaryotics bacteria and archea- and eukaryotics such as fungi (yeat, moulds) , protoza, and algae.

loader
I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
capcha
Download Presentation

PowerPoint Slideshow about 'In the name of God' - LeeJohn


An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript
In the name of god l.jpg

In the name of God

Diagnostic microbiology


Background l.jpg
Background

  • Microbiology is the study of cellular and acellular cells and agents.

  • Cellular micro-organisms are consisted of prokaryotics bacteria and archea- and eukaryotics such as fungi (yeat, moulds) , protoza, and algae.

  • In regard to their size, smallest bacteria such as mycoplasma are 0.1-0.3 and the largest eukaryotic such as helminth have diameter of >1000


Slide3 l.jpg

  • Acellular infectious agents are nonliving structures. Some have nucleic acids either DNA and/or RNA

  • Some have no any nuclear elements and are just protein in nature prions

  • Some of these acellular agents are toxins produced by bacteria and fungi.

  • Some bacterial products or fungal metabolites are beneficial and are antimicrobial agents.

  • Microbial toxin are metabolite which can cause intoxication rather than infection.


Acellular infectious agents l.jpg
Acellular infectious agents: have nucleic acids either DNA and/or RNA

  • Perions : are structurally deformed proteins, when they change in tertiary configuration they can invade neural cells. Prions are the causative agents of scrapies in sheep, mad cow disease in cows and cattle and spongy form encephalitis in human.

  • Nanobacteria : are newly diagnosed and very small bacterial form of life.

    these are assumptions about their role in renal stone formation and some form of atherosclerosis.

  • Toxins : are microbial substances that are able to induce damage to host cells, an immunogenic or allergenic response and/or fever such as

    • Endotoxins of G(-) bacteria

    • Neurotoxins of Cl-Tetani

    • Entrotoxins of E-coli vibriocholera

    • Aflatoxins of asepergillus rubratoxin

    • Algal toxin such as saxtoxin and hepatotoxin


Slide5 l.jpg


Slide6 l.jpg

  • Microbes that can cause infectious disease are called pathogens. Some of them are true pathogens meaning that when they colonies to special site or receptors they cause infectious disease, like Niesseria gonorhea and shigella species etc,… and some of them produce disease under certain conditions, opportunistic like pseudomonas spp and many others.


Slide7 l.jpg


What are virulence factors l.jpg
What are virulence factors: to:

  • Virulence factor are:

    • cell structure component : LPS of the G(- )bacteria

    • cell structure appendages : pili, resistance genes on plasmids etc,…

    • Capsule : which inhibit phagocutosis, capsule in strep pneumonia, bacillus antheracis

    • Slime layer production : exopolysaccharide production by a mixture of organisms biofilm production

    • Microbial toxins : diphtheriatoxin, cholera toxin


Slide9 l.jpg

  • Some of these virulence factors are inherited, capsule production, cell destructive enzymes. (Leokocidin, Hemolysins, DNAes, RNAes, Coagulase, phospholipase, Lipase, etc,…)

    and some of these virulence factors may be acquired by genetic phenomena like transformation, genetic transduction (Scarlet fever toxin, diphtheria toxin and genetic conjugation, transfer of resistant factors.)


Slide10 l.jpg

  • Some microbes are able to live in extracellular environment and cause an acute pus forming infectious disease such as staph, aureus, strep pyogens coryrebacterium diphtheria, Neissena meningitidies Neissena gonorrlea etc,…

  • Some bacteria should live intracellularly in order to cause disease such as chlamydia causative agents of trachoma, preumonia, and genital infections.

  • Ehrlichia specis which produce disease when they multiply within macrophages causing disease known as human monocythic Ehrliosis.

  • Anaplasma phagocytophilnm live within granulocytes plasmodium Spp(malaria) and baesia which produce intra erythrocytic disease.


Slide11 l.jpg

  • Some microbes are facultative in respect to disease production such as leishmania specis, M.TB, listeria monocytogenes.

  • Some microbes such as Brucella species, Salmunella species, Yersinia pestis, fancisella can alive intracellulary.

  • They produce some enzyme, which combat with intracellular killing mechanisms.


Slide12 l.jpg

  • Some algae also can cause infection such as genus prototheca. Prototheca live in soil and decaying matter. They can enter wound especially in foot. they can also invade lymphatic and produce systemic and fatal infection. Prototheca can be grown in the laboratory, they produce yeastlike, colonies.


Slide13 l.jpg

  • Clinically important fungi and moulds prototheca. Prototheca live in soil and decaying matter. They can enter wound especially in foot. they can also invade lymphatic and produce systemic and fatal infection. Prototheca can be grown in the laboratory, they produce

    dermatophytes: which produce infection in hair, nail and skin such as epidermophyton trichophyton, candida yeast.

    Fungi which can cause mucosal infection such as mucromycosis, asperiogillus

    Fungi which can cause systemic infection such as yeast, histoplasma capsulatum etc,…


Slide14 l.jpg

  • Clinically important prototheca. Prototheca live in soil and decaying matter. They can enter wound especially in foot. they can also invade lymphatic and produce systemic and fatal infection. Prototheca can be grown in the laboratory, they produce protozoa

    Malaria: plasmodium species

    Leishmania species (kala-azar, mucocutonous infection)

    Amoeba: E-histolytica

    Ciliate: Balantidium coli

    Flagellate: Trichomonas, Giardia

    Helminths

    crypthosporidium

    etc,…


Slide15 l.jpg

  • Clinically important viruses prototheca. Prototheca live in soil and decaying matter. They can enter wound especially in foot. they can also invade lymphatic and produce systemic and fatal infection. Prototheca can be grown in the laboratory, they produce

    • Hepatits viruses, such as HBV, HAV, HCV, HDV, HEV, HGV

    • HIV and related immunodefeciency viruses HIV1 and HIV2

    • Herpes simplex and related viruses such as varicellazoster virus, VZV, cytomegalovious CMV

    • Epsteinbar virus EBV

    • Crimian congo hemorhagic fever virus CCHFV

    • Ebola viruses

    • Influenzae viruses, Entero viruses and many others.


Slide16 l.jpg

  • Acellular proteinous structure causing infecious disease prototheca. Prototheca live in soil and decaying matter. They can enter wound especially in foot. they can also invade lymphatic and produce systemic and fatal infection. Prototheca can be grown in the laboratory, they produce

    Prions: prions are protein in naturre. When their tertiary structure is change due to mutation, it becomes infectious.

    Prions are assumed to be the cause degerative disease of nerve cells.

    prions are the causative agents of certzfeid Jacob syndrome in human.

    Madcow disease in cow and cattles.

    Scrapies in sheep and goats.


Slide17 l.jpg

Optimal times for specimen collection prototheca. Prototheca live in soil and decaying matter. They can enter wound especially in foot. they can also invade lymphatic and produce systemic and fatal infection. Prototheca can be grown in the laboratory, they produce

In the suspected case of salmonellosis typhoid and paratyphoid-take a blood culture in the first week of infection.

Serology test in the end of the second week and urine and stool culture during the second and third week may show positive result.


Slide18 l.jpg

In case of bacteremia and sepsis blood is taken prototheca. Prototheca live in soil and decaying matter. They can enter wound especially in foot. they can also invade lymphatic and produce systemic and fatal infection. Prototheca can be grown in the laboratory, they produce

for culture in order to isolate the suspected

organism.

In every case try to obtain blood for culture before

antibiotic therapy. If this is not unavoidable try to

use culture media which contains resins to bind to

the suspended antibiotics.


Slide19 l.jpg

Specimen collection for microbiology examination prototheca. Prototheca live in soil and decaying matter. They can enter wound especially in foot. they can also invade lymphatic and produce systemic and fatal infection. Prototheca can be grown in the laboratory, they produce

Once an infectious disease is suspected and after

physician request for specimen collection all

quality criteria should carried out.

Although culturing and isolation of the infecting

microbe is the gold standard there are situations in

which the pathogen can’t be isolated by routine

culturing methods and media.


Slide20 l.jpg

This may be due to the bacterium or microbe in prototheca. Prototheca live in soil and decaying matter. They can enter wound especially in foot. they can also invade lymphatic and produce systemic and fatal infection. Prototheca can be grown in the laboratory, they produce

state of VBNC meaning that although the bacteria

are alive (viable) but nonculturable.

The change in the PH of infected site may cause decrease

in viability and although presence of many enzymes

produced by the inflammatory cells (peroxidases,

phospholipases, RNAes, DNAes) may result in

destruction of microbes.


Slide21 l.jpg

Prior antibiotic therapy and incomplete dose may be another cause in which although the disease is yet present but the infecting agent is inactivated.

In the case of wound infectious first cleans the superficial pus and dirt by alcohol and then if the wound is open draw the specimen from edge of the infection sites without touching the adjacent skin.


Slide22 l.jpg

In the case of cervical and endometrial culture don’t contaminate the specimen with vaginal secretions.

In the case of UTI suspected patient, the best way is to obtain a clean cached early morning mid stream urine or any urine remained in the bladder at least for 4 hours.


Slide23 l.jpg

Taking urine specimen by catheters is not advised unless other wise there is no any simpler way.

Suprapubic urinecollection should be remained for very small children in which collection of CVU is not possible to them.

In case of streptococcal pharynsitisthe peritonsillar fossae and postpheraryngeal wall is swabed


Slide24 l.jpg

In case of lower respiratory tract infection try to obtain the sputum by a deep cough.

It is better that patient have had a mouth wash and after that a deeply coughed sputum be taken in wide mouth sterile container.

In the case of any deeply seated abcess or pus loculation, the best way of specimen collection is by aspiration.


Slide25 l.jpg

In the case of sinusitis the best specimen is obtained through suctioning of sinus discharge, otherwise with a flexible sterile swab via the nostriles enter the sinus.

In the case of otitis media, carefully enter a sterile swab toward the site of infection.

In the case of meningitis- after LP done by physician transport the tube, as soon possible and centrifuge the content of the second tube for culturing and smear .


Slide26 l.jpg

Improper specimen collection through suctioning of sinus discharge, otherwise with a flexible sterile swab via the nostriles enter the sinus.

This is a physician or an oriented HCW to take specimen from the actual site of infection

As it is possible do not mix the specimen with micriflora present in the site.

As it is possible try to take specimen before any chemotheraputic (antibiotic) are used.


Slide27 l.jpg

As it is possible try to take sufficient amount of specimen quality and quantity of specimen is of importance.

As soon as possible transfer the specimen to the lab, in a safe manner


Slide28 l.jpg

Rejection criteria in CML specimens quality and quantity of specimen is of importance.

  • Reject specimen taken in broken or leaky container

  • Reject voided urine, vomitous for an aerobic culture.

  • Reject saliva and mouth wash as sputum specimen.


ad