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?????????? . ???????????????????????????????????? Mitragyna speciosa Korth. ?????? Rubiaceae??????? (????????????????) ???????? ??????????? ??????????. ??????????????????????. ???????????????????? ???? ???????????????????????????? 5 ??? ?.?.? ?????????????? (??????? 5) ?.?. 2545 ???????? ???? ???????? ??????? ???????????? ?????????????????????????????????????????????????? ?????? 9 ???????????? ???????????????????????????????????????? 1 ?.?. 2548 ??????????????????????????????????????????224

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    1. ????????????????????????????? ??. ??????? ???????????? ????????????????????? ?????????????????????? 7 ?.?. 2551

    3. ?????????????????????? ???????????????????? ???? ???????? ???????????????????? 5 ??? ?.?.? ?????????????? (??????? 5) ?.?. 2545 ???????? ???? ???????? ??????? ???????????? ????????????????? ????????????????????????????????? ?????? 9 ???????????? ???????????????????????????????????????? 1 ?.?. 2548 ????????????????????????????????? ???????????????????????? 9: ?????????????????????????????????????????????????????????????????????

    4. ???????????? ???????????????????????????????????????????? ?????????? ?????? ??????????? ??????????????????? ??????????? ???????????????????????????????????????????????????? ?????????????????????????????????????????????????????

    5. ??????????????? ????????????????????????????? ????????????????????????????? ???????????????? ?????????? ??????????????????????????????? ???????????????????????????????????? ??????????????? ????????????? ???????????????????????? ??????? ????????????????????? ?????????? ????????????? ????????????? ?????????????? ??????????????? ??????????????????????????? ??????????????????????

    6. ???????????????????? ????????????????????????????????????????????????? ???? ????? ?????? ??????? ??????? ??????????????????????????????????????

    7. ??????????????/???????????????? ???????-???????-????????? ???????-???????-?????? ???????-??????????-????????? ??????????????

    8. ?????????????????????????????????

    9. Mitragynine ?????????????????????????????????????????????????? ??????????????????????????????????? ?????????????????????? 0.5 ???????????? ??????????? 66.2 ??? crude alkaloid extract ???????????? speciogynine (?????? 6.6) speciociliatine (?????? 0.8) paynantheine (?????? 8.6)

    10. ???????????? Mitragynine ??? Isomers

    11. Possible m/z fragments from Mitragynine type Alkaloids

    12. Mass fragments of Mitragynine type Alkaloids

    13. Possible m/z fragments from Corynantheidine type Alkaloids

    15. Possible m/e fragments from Oxyndole type Alkaloids

    17. ??????????????????????????? ???????????????????????????? ?????????????????/??????????????? ???

    18. Solid Phase Extraction DSC-MCAX SPE (Supelco):100 mg x 3ml Mixed-mode cation exchange

    21. ??????????????????????????????????????????????

    22. Mitragynine STD

    23. ????????????? 4 ?????????????????????????

    24. ????????????? 4 ?????????????????????????????????????

    25. Mass fragments of the 4 alkaloids

    26. ????????????? 2 ???????????????????????????? 4x100

    27. ????????????? 4 ???? ??????????????????????????????????? ????????????????????????????????? 1. 9-Methoxy Oxyndole Alkaloid: RT 12.179 (M 414) 2. Mitragynine: RT 14.777(M 398) 3. Paynantheidine: RT 14.979(M 396), 4. Speciogynine: RT 15.297(M 398)

    28. ????????????? 2 ???? ???????????????????????? 4x100 1. 9-Methoxy Oxyndole Alkaloid: RT 12.179 (M 414) 2. Mitragynine: RT 14.777(M 398)

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