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Bachelor-, Studien- Diplomarbeit Beginn ab November 2008 möglich. Ansprechpartner: Dipl.-Biol. Julika Wrenger Tel.: 0531/391-5746 Email: j.wrenger@tu-bs.de. Arbeitsmethoden:. PCR Klonierung Transformation (Elektroporation) Kultivierung im 3 L Reaktor Gelpermeationschromatografie.

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Bachelor-, Studien- DiplomarbeitBeginn ab November 2008 möglich

Ansprechpartner:

Dipl.-Biol. Julika Wrenger

Tel.: 0531/391-5746

Email: j.wrenger@tu-bs.de

Arbeitsmethoden:

PCR

Klonierung

Transformation (Elektroporation)

Kultivierung im 3 L Reaktor

Gelpermeationschromatografie

Institut für Biochemie und Biotechnologie

Abteilung Biotechnologie

Thema:

Klonierung einer Beta-Glucanase aus dem Pilz Trichoderma harzianum. Anschließend erfolgt eine Kultivierung im Expressionssystem E. coli. Test der Enzymaktivität am Pilz-Polymer Scleroglucan inklusive Strukturanalyse mittels GPC und 13C-NMR