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Understanding Aminoacyl-tRNA Synthetase: Mechanisms, Kinetics, and Applications

This paper explores the critical role of aminoacyl-tRNA synthetase (aaRS) in the translational process within living cells, specifically in ribosomes. It details the mechanism of tRNA and amino acid binding, enzyme kinetics, and factors influencing enzyme activity such as temperature, substrate concentration, and inhibitors. The paper also discusses potential applications of enzyme inhibitors in treating diseases like Malaria and HIV, as well as their role in antibiotic development. Special attention is given to serine-tRNA ligase and its unique characteristics.

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Understanding Aminoacyl-tRNA Synthetase: Mechanisms, Kinetics, and Applications

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  1. Aminoacyl tRNA synthetase (aaRS) Oleh: Ertanti Rizki P. 20512033 Suci Rizki Nurul Aeni 20512044 KK Biokimia-Institut Teknologi Bandung

  2. Outline

  3. Terdapat dalam sel makhluk hidup, yaitu pada Ribosom What is aa RS ? Berperan dalam proses translasi RNA Pengikatanasam amino spesifikketRNA

  4. Tahapan pengikatantRNAdenganasamamino • Sisiaktifenzimmengikatasam amino dan molekulATP. 2) ATP kehilanganduagugusfosfatdan bergabungdenganasam amino sebagaiAMP. 3) tRNAberikatankovalendenganasam amino menggeserAMP disisiaktifenzim. 4) EnzimmelepaskantRNA-aminoasil, disebut juga“asam amino teraktivasi”

  5. Mekanisme

  6. Perbedaan Kelas Asam Amino

  7. Kinetics

  8. kineticsof Enzyme • Suhu • Konsentrasi enzim • Konsentrasi Substrat • Inhibitor • Jumlah Pyrophosfat

  9. What are development? • Inhibitor enzim ini dapat berguna bagi treatment untuk beberapa penyakit antara lain Malaria dan HIV 1 • Inhibitor enzim ini berpotensi untuk zat antibiotik

  10. Spesific Enzyme Name of IUBMB • EC 6 : Class Ligase • EC 6.1 : Subclass Forming Carbon-Oxygen Bonds • EC 6.1.1 : Ligases forming aminoacyl-tRNA and related compounds • EC 6.1.1.11 : Serine-tRNAligase • Nama lain : seryl-tRNA synthetase; SerRS; seryl-transfer ribonucleate synthetase; seryl-Transfer RNA synthetase; seryl-transfer ribonucleic acid synthetase; serine translase • NamaSistematik: L-serine:tRNASer ligase (AMP-forming)

  11. Function of Serine-tRNAligase • Mengakatalisispengikatankovalendariserinkegugus 3’-OH darinukleotidatRNA-nyadalamsuatuproses yang digerakanolehhidrolisis ATP.

  12. Reaction of Serine-tRNAligase ATP + L-serine + tRNASer AMP + diphosphate + L-seryl-tRNASer

  13. Characteristic of Serine-tRNAligase • Terdapatdua domain yang terikat pada active side dan pada antikodon • Domain N-terminal terdiridarirantai yang sangatpanjang • Seryl-tRNAsynthetasememilikisetidaknya lima spesies yang berbeda yang secarakolektifmengakuienamkodonberbedauntukasam amino

  14. Kineticsof Serine-tRNAligase

  15. Applicationsof Serine-tRNAligase

  16. Modificationsof Serine-tRNAligase

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