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Comparative Analysis of Synthetic Promoters in Rat Hepatoma Cells

Assessment of luciferase activities for 3-element and 6-element synthetic promoters in rat hepatoma cells compared to CMV promoter activity. The study involved transfer of modules to luciferase reporter vector and luciferase assay. Promoter activity calculated as a percentage of CMV activity.

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Comparative Analysis of Synthetic Promoters in Rat Hepatoma Cells

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  1. a b Fig. S2. Luciferase activities of synthetic promoters in rat hepatoma cells. (a) Activities of 3-element synthetic promoters. All 3-element modules were transferred to a luciferase reporter vector generating 3-element SP-Luc, followed by luciferase assay. (b) 6-element synthetic promoters that had more than 7.5% of CMV promoter activity. Luciferase activities of synthetic promoter constructs were calculated as percentage of CMV promoter activity. All values are means ± S.D. L-PK, pLPK-Luc plasmid; h, HNF-1 binding element; c, C/EBP binding element; g, glucose-responsive element.

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