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Overview of LacZ Cosmid Vector Sequence and Structural Features

This document provides a detailed examination of the LacZ cosmid vector (designated A.Ampr.2464.bp.T3.T7.B) with an expanded view of its regions. The total length, including the expanded region, is approximately 43 kb. The vector features include specific lambda right and left arms, with lengths of ~19 kb and ~24 kb respectively upon EcoRI digestion. The vector's ends are structurally identical to those of vector B, though represented schematically. Key restriction sites include XbaI, EcoRI, HindIII, and NotI for further genetic manipulation.

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Overview of LacZ Cosmid Vector Sequence and Structural Features

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  1. A Ampr (2464 bp) T3 T7 B Expanded view of region within vector LacZ cos cos Total length including expanded region = 43kb Lambda left (to EcoRI) = ~19 kb Lambda right (from EcoRI) = ~24 kb C cos cos Total length including central stuffer = 43kb The ends of the vector shown here are identical to those of vector B. They are just schematized differently. Vector Page XbaI→ EcoRI→ HindIII→ Not I→

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