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Unit 8. Diffusion and Osmosis. Exercise 8.1 p 109. Examine two factors that affect the rate of diffusion Temperature Molecular weight We will look at the movement of two dyes as they diffuse through an agarose gel. 8.1. 1.Obtain petri dishes with agarose.

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unit 8

Unit 8

Diffusion and Osmosis

exercise 8 1 p 109
Exercise 8.1p 109
  • Examine two factors that affect the rate of diffusion
    • Temperature
    • Molecular weight
  • We will look at the movement of two dyes as they diffuse through an agarose gel.
slide3

8.1.

1.Obtain petri dishes with agarose.

2. Punch out 2 wells with a straw. This is to make a depression in which to place the dye.

3. Put a drop of potassium permanganate in one well and a drop of Janus Green in the other well.

Photo: Jeff Beck, CCCCD

4. Incubate one plate at RT and one plate on ice.

5. At 15 minute intervals measure the zone of diffusion around each well (Measure diameter of the ring)

osmosis
Osmosis
  • Specialized type of diffusion involving diffusion of water molecules.
  • Movement of water molecules from an area of higher water concentration to an area of lower water concentration.
dialysis tubing
Dialysis tubing
  • Differentially permeable material
  • Has certain pore size that only allows molecules smaller than the pore to pass through.
  • Larger molecules cannot pass through.
  • Dialysis tubing simulates the plasma membrane.
  • Inside the bag- inside of cell
  • Fluid in beaker- outside of cell
ex 8 2 effect of solute concentration on osmosis
Ex. 8.2 Effect of solute concentration on osmosis
  • Dialysis tubing will be used to simulate movement of substances through cell membranes
slide7

Prepare tubing by tying one end with string, pipetting liquid into bag, then tying other end with string.

Trim off excess string.

Photo: Jeff Beck, CCCCD

slide8
Set up 5 bags and 5 beakers with the solutions described in the lab manual.
  • Weigh bags before placing in solution in beaker.
  • Re-weigh bags at 20 minute intervals (pat bags dry before weighing)
  • Determine which bags gained or lost weight.
  • Were bags placed in iso-, hypo-, or hypertonic environments?
ex 8 3 differential permeability of membranes
Ex 8.3 Differential permeability of membranes
  • The dialysis tubing contains microscopic pores of a certain size.
  • Only molecules small enough to go through pores can pass through the membrane.
  • Set up a beaker with a dialysis bag using the solutions as described in the manual.
slide10
Incubate for 1 hour, then test the beaker contents for
    • Starch (iodine test)
    • Chloride ion (silver nitrate test) (Special disposal)
    • Sulfate ion (Barium chloride test) (Special disposal)
    • Protein (Biuret test)
  • Which molecules passed through the tubing and into the beaker?
  • Why do you think some molecules but not others passed through the tubing?
diffusion osmosis in living cells
Diffusion/Osmosis in Living Cells
  • 8.4A Osmosis with Red Blood Cells
  • Demo microscopes set up in back for you to view and draw what you see in Table 8.5
osmosis in red blood cells

Isotonic

Hypertonic

Hypotonic

Osmosis in Red Blood Cells
  • Observe sheep RBCs via a wet mount of the sample

Crenation

Cells swell and may burst.

http://www.msu.edu/~butter12/BS111L/Diffusion%20and%20Osmosis.ppt#263,13,Osmosis in Plant Cells

slide13

RBC in isotonic solution

    • Plump round cells

Photo: Jeff Beck, CCCCD

slide14

Red blood cells in hypertonic solution

Crenate (spiky, not smooth edge)

Photo: Jeff Beck, CCCCD

slide15
RBCs in hypotonic solution
    • Cells may not appear as they have burst
  • View a page of text through each of the tubes of blood. Can you read the text?
osmosis with plant cells ex 8 4b
Osmosis with plant cellsEx 8.4B
  • Instructor will set up wet mount demo of Elodea leaves
  • One will be soaked in hypertonic (20% saline) solution
  • One will be soaked in distilled water (hypotonic).
  • Definition of turgor pressure is the pressure of the cell contents against the cell wall.
plant cells in hypertonic solution
Plant cells in hypertonic solution
  • Plant cells have rigid cell walls
  • Vacuole will lose water and plasma membrane will pull away from the cell wall. This is called plasmolysis.
plants in hypotonic solution
Plants in hypotonic solution
  • Vacuoles will be filled with water. This cell exhibits turgor.
elodea cell in hypertonic and hypotonic solutions
Elodea cell in hypertonic and hypotonic solutions
  • Hypertonic-
    • Chloroplasts will be concentrated at the center of the cell.
    • Why?
  • Hypotonic-
    • Chloroplasts will be distributed around the edge of the cell, or distributed evenly throughout. (Not concentrated in one place)
    • Why?
osmosis in plant cells

Hypertonic

Hypotonic

Osmosis in Plant Cells

Plasmolysis

  • Observe Elodea leaves via a wet mount of the sample
  • Note location of central vacuole and chloroplasts in each sample

http://www.msu.edu/~butter12/BS111L/Diffusion%20and%20Osmosis.ppt#263,13,Osmosis in Plant Cells

slide21

Animation of plasmolysis

http://ccollege.hccs.edu/instru/biology/AllStudyPages/Diffusion_Osmosis/Elodeagif.swf

Photo: Jeff Beck, CCCCD