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Dr. Sonia Siddiqui Dr. Panjwani Centre For Molecular Medicine and Drug Research (PCMD)

Core Course MM # 707 Electrophysiology: Patch Clamp. Dr. Sonia Siddiqui Dr. Panjwani Centre For Molecular Medicine and Drug Research (PCMD). 3T3 cells. 3T3 cells. Butanol extracts (10 ug/ml). Butanol extracts (20 ug/ml). Control.

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Dr. Sonia Siddiqui Dr. Panjwani Centre For Molecular Medicine and Drug Research (PCMD)

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  1. Core Course MM # 707 Electrophysiology: Patch Clamp Dr. Sonia Siddiqui Dr. Panjwani Centre For Molecular Medicine and Drug Research (PCMD)

  2. 3T3 cells

  3. 3T3 cells

  4. Butanol extracts (10 ug/ml) Butanol extracts (20 ug/ml) Control Results: Effect of cardamom extracts (Butanol) on 3T3 cell line Original recording graphs showing the same duration (100 msec) of the opening of the channels in B1 and B2 when compared to controls. However B2 increases outward rectifying potassium currents

  5. Results: Effect of cardamom extracts (D1-D3 hydroalcoholic phase) on 3T3 cell line D1 (10 ug/ml) D2 (15 ug/ml) D3 (20 ug/ml) C

  6. Results: Effect of cardamom extracts (EthylAcetate) on 3T3 cell line EA2 EA1 C

  7. Results: Effect of cardamom extracts (n-Hexane) on 3T3 cell line C n-Hexane-1 n-Hexane-2

  8. 60mV -55mV /holding potential -110mV -60mV Postnatal Hippocampal neurons Identification of hippocampal neurons Pattern of hippocampal firing Pattern of cortical neurons firing Effect of size of the cell body of a neuron Effects from Differentiated neurons on the currents Identification of 3T3 cell line Pulse Protocol for Na and K+ currents

  9. Results: Hippocampal neurons

  10. After 5 mins C After 10 mins Results: Effect of peptide (P1 ) on Cortical neurons

  11. After 5 mins After 10 mins C Results: Effect of peptide (P2 ) on Cortical neurons

  12. After 5min After 10min Control Results Effect of peptides isolated from bacteria on cortical neurons

  13. Results: Effect of peptide (P3) on Hippocampal neurons K+ channels After 5 mins After 10 mins C K+ channels After 10 mins After 5 mins C

  14. Results: Effect of peptide (P4 ) on hippocampal neurons

  15. Results: Methonolic NicotinumTobacom Extract (0.5mg/ml) Control NA Currents were normalized by cell capacitance (pA/pF) Methonolic Extract was tested on hippocampal cultured neurons. We found that it blocks delayed rectified Potassium currents

  16. Na currents 2 Results CentellaAsiatica Extract (0.5mg/ml)On K+ and Na+ Currents CA Control Asiaticoside, an active constituent in methanol and ethylacetate extracts of C. asiatica, has anxiolytic activity. The extracts of C. asiatica, except Aqueous extract, possess anticonvulsant and neuroprotective activity and thus can be used for effective management in treatment of epileptic seizures. Methonolic Extract was tested on hippocampal cultured neurons. We found that it does not effect delayed rectified Potassium currents CA Control Control Control CA Na currents

  17. Results CentellaAsiatica Extract (0.5mg/ml) On Sodium Currents Control CA Wash Methonolic Extract was tested on hippocampal cultured neurons. We found that it reduces Sodium currents. This is in consistent with the previous reported studies by Visweswari et al., 2011. This medicinal herb used as an anticonvulsant and for enhanced memory. The most important ingredients in methonolic extract is Asiaticoside which has an anxiolytic activity.

  18. Results CentellaAsiatica Extract (0.5mg/ml) On Sodium Currents

  19. Results: SerpindusTrifalitusextract (0.5mg/ml) On hippocampal K Currents Transient K Currents Sustained K Currents Control ST

  20. Results: SerpindusTrifalitusextract (0.5mg/ml) On hippocampal Na Currents ST Control We observed that after 2-3 min application of Serpindus Trifalitus extract all cells were died in culture plate

  21. Results: Co-cultured Hippocampal neurons

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