1 / 2

Comprehensive Sequence Analysis of Catrocollastatin-C from Western Diamondback Rattlesnake Venom

This summary presents the detailed sequence analysis of Catrocollastatin-C, a 23.6-kD protein sourced from the venom of the Western Diamondback Rattlesnake (Crotalus atrox). The overall amino acid sequence consists of 216 residues, derived from overlapping sequences obtained through various methods such as Edman degradation, CNBr cleavage, and endopeptidase Lys-C digestion. Each method contributed unique peptide fragments that were sequenced to identify overlaps, resulting in a comprehensive analysis of this biologically significant venom protein.

yvonne
Download Presentation

Comprehensive Sequence Analysis of Catrocollastatin-C from Western Diamondback Rattlesnake Venom

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Summary of the sequence analysis of catrocollastatin-C,a 23.6-kD protein found in the venom of the western diamondback rattlesnake Crotalus atrox.Sequences shown are given in the one-letter amino acid code.The overall amino acid sequence(216 amino acid residues long)for catrocollastatin-C as deduced from the overlapping sequences of peptide fragments is shown on the lines headed CAT-C.The other lines report the various sequences used to obtain the overlaps.These sequences were obtained from(a)N-term:Edman degradation of the intact protein in an automated Edman sequenator;(b)M:proteolytic fragments generated by CNBr cleavage,followed by Edman sequencing of the individual fragments(numbers denote fragments M1through M5);(c)K:proteolytic fragments(K3 through K6)from endopeptidase Lys-C cleavage,followed by Edman sequencing;(d)E:proteolytic fragments from Staphylococcus protease(E13 through E15)digestion of catrocollastatin sequenced in the Edman sequenator.

More Related