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Lab-on-a-chip Devices for Cell Separation and Identification. Feb 2008. A PhD project at Macquarie University under the guidance of Dr. David Inglis and Prof. Ewa Goldys. Microbes, cells and small organisms come in a wide array of shapes and sizes. Yeast. Blood Cells.

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lab on a chip devices for cell separation and identification

Lab-on-a-chip Devices for Cell Separation and Identification

Feb 2008

A PhD project at Macquarie University under the guidance of Dr. David Inglis and Prof. Ewa Goldys.

microbes cells and small organisms come in a wide array of shapes and sizes
Microbes, cells and small organisms come in a wide array of shapes and sizes

Yeast

Blood Cells

Photo Credit: H.D.A Lindquist, U.S. EPA

cryptosporidium

microfluidic or lab on a chip devices can be used to separate different cell types
Microfluidic or Lab-on-a-chip Devices can be used to separate different cell types
  • Various blood cell separations have been demonstrated:
  • J. A. Davis, D. W. Inglis, K. M. Morton, D. A. Lawrence, L. R. Huang, S. Y. Chou, J. C. Sturm, and R. H. Austin, Deterministic hydrodynamics: taking blood apart, Proc. Nat. Acad. Sci. (USA), vol. 103, pp. 14779-14784, 2006.
  • D. W. Inglis, J. A. Davis, T. J. Zieziulewicz, D. A. Lawrence, R. H. Austin, and J. C. Sturm, Determining blood cell size using microfluidic hydrodynamics, J. Immunol. Meth., vol. 329, pp. 151-156, 2008.
  • D. W. Inglis, K. J. Morton, J. A. Davis, T. J. Zieziulewicz, D. A. Lawrence, R. H. Austin, and J. C. Sturm, Microfluidic device for label-free measurement of platelet activation, Submitted to Lab on a Chip, Jan 08.
  • But no work yet with microorganisms!
slide4

This project will lead to publications which demonstrate the separation of microorganisms on portable inexpensive polymer microfluidic devices.

Such devices may have applications in

  • early detection of human diseases such as sepsis and various parasitic diseases
  • as well as management of swimming pool and drinking water quality.
what is micro fluidics
What is Micro-Fluidics

Fabrication Process:

Photolithography

  • Microfluidics –microfabricated structures for fluid handling
  • Engineers know how to microfabricate and build integrated systems
  • mTAS – Micro Total Analysis System
    • Integrate microfluidic components into a useful device, a “Lab on a Chip”
  • “development and application of micro- and nanofabricated devices and systems for chemical and biochemical measurements. The technology encompasses extensive areas of application in clinical diagnostics, genomics and proteomics, environmental assays, separation science, cellular analysis or drug discovery.” From Micralyne.com
  • Interdisciplinary – Physics, Chemistry, Biology

Reactive Ion Etching

Drill access holes

Seal with cover slip

Fluid goes in

high resolution separation with size position correlation

1.4 mm

1 mm

1.5 mm

1.6 mm

200 mm

1.7 mm

1.8 mm

1.9 mm

2.0 mm

2.1 mm

2.2 mm

High Resolution Separation with Size - Position Correlation
  • Peak width ~10 nm
  • No known method fractionates with such resolution.

Can include chirp to fractionate over a range of sizes by varying

gor

Huang et al, Science 2004

white blood cells come in different sizes and can be differentiated
White blood cells come in different sizes and can be differentiated

~

Cancerous

Lymphocytes

Healthy

Lymphocytes

proposed device
Proposed Device
  • Use the technique described above to create a simple to use high throughput cell concentrator.
  • Parallel channels for separation and concentration on a glass slide. A second layer distributes fluid to the channels and collects their output.
  • Structures will be molded PDMS.
  • Master mold will be lithographically defined SU-8 polymer.

20-micron PDMS pillars