1 / 15

RNA interference:

RNA interference:. Kill the messenger!. Discovery. inject worms with dsRNA corresponding to a gene involved in wiggling ( unc-22 ). conclusion: dsRNA triggers potent and specific gene silencing. P. 21 nt long, dsRNA:. P. Biochemistry to Examine Structure. short interfering RNA: siRNA.

yin
Download Presentation

RNA interference:

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. RNA interference: Kill the messenger!

  2. Discovery inject worms with dsRNA corresponding to a gene involved in wiggling (unc-22) conclusion: dsRNA triggers potent and specific gene silencing

  3. P 21 nt long, dsRNA: P Biochemistry to Examine Structure short interfering RNA: siRNA

  4. Mix cell extract with dsRNA RNase III endonuclease 21-mer Berstein et al.Nature (2001) Dicer generates siRNAs

  5. dsRNA siRNA RISC cap cap mRNA mRNA AAAA AAAA RNA interference (RNAi) Dicer

  6. short RNAs identified in plants RNAi shown in vitro siRNAs identified Dicer identified genome-wide RNAi screens begin RNAi used against HIV Timeline 1990 cosuppression of purple color in plants dsRNA injection in worms 1998 1999 RISC activity partially purified 2000 2001 2002

  7. Big Potential for Small RNAs Adapted from Ryther et.al. Gene Therapy (2005)

  8. S2 Drosophila cells spread on coated glass surface via lamellipodia

  9. Example of RNAi experiment

More Related