GA-repeat (aa88-323)

1. msdegpgtgpgnglgekgdtsgpegsggsgpqrrggdnhgrgrgrgrgrgggrpgapggs gsgprhrdgvrrpqkrpscigckgthggtgagagaggagaggagagggagagggaggagg aggagagggagagggaggaggagagggagagggaggagagggaggaggagagggagaggg aggagagggaggaggagagggagaggaggaggagaggagagggaggaggagaggagagga gaggagaggaggagaggaggagaggaggagagggaggagaggaggagaggaggagagga ggagaggaggagagggagaggagaggggrgrggsggrgrggsggrgrggsggrrgrgrer arggsrerargrgrgrgekrprspssqssssgspprrpppgrrpffhpvgeadyfeyhqe ggpdgepdvppgaieqgpaddpgegpstgprgqgdggrrkkggwfgkhrgqggsnpkfen iaeglrallarshverttdegtwvagvfvyggsktslynlrrgtalaipqcrltplsrlp fgmapgpgpqpgplresivcyfmvflqthifaevlkdaikdlvmtkpaptcnirvtvcsf ddgvdlppwfppmvegaaaegddgddgdeggdgdegeegqe C-terminal (aa324-641) N-terminal (aa1-87) GA-repeat (aa88-323) E-figure 1. The three main domains of EBNA1 (N-terminal, GA-repeat and C-terminal) are outlined in the primary protein sequence. One epitope (aa 393-407: PPRRPPPGRRPFFHP) located within in the C-terminal domain that was preferentially recognized by MS patients’ sera is underlined in the EBNA1 primary protein sequence. However, additional specificities of EBNA1-targeting IgG in children with MS recognized epitopes throughout the protein and were not restricted to a unique region or domain of EBNA1.