UCSC Known Genes Version 3 Take 10. Overall Pipeline. Get alignments etc. from database Remove antibody fragments Clean alignments, project to genome Cluster into splicing graph Add EST, Exoniphy, OrthoSplice info. Walk unique transcripts out of graph.
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Snap soft ends to hard
Comparison vs. RefSeq reviewed ORF annotations.
*twinOrf only predicts if has homologous sequence. This run with dog,
only adds up to 97.2% for this reason.