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This study explores the functional role of TFII-I protein in gene regulation using multiple experimental techniques such as western blotting and immunoprecipitation. The findings shed light on the mechanism of action of TFII-I in gene expression.
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+/+ +/Dex2 TFIIGST TFII-I IgG TFIIV5 DTFIIV5 IP: V5 Western: GST PARP-GST Western: V5 A B TFII-I TFII-I input input Input TFII-GST/DTFIIV5 IgG IgG IP TFIIGST/DTFIIV5 Input TFIIGST/TFIIV5 IP TFIIGST/TFIIV5 IgG IP: V5 Western: GST +/+ +/Dex2 C Dex2/ Dex2 Birc1F Renl