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HPLC Phytoplankton Pigment Measurements: Continuation of Climate Quality Data Records (CQDR) in Support of NASA’s Earth

HPLC Phytoplankton Pigment Measurements: Continuation of Climate Quality Data Records (CQDR) in Support of NASA’s Earth Science Enterprise (ESE) C.C. Trees and J.L. Mueller Center for Hydro-Optics and Remote Sensing (CHORS) San Diego State University (ctrees@chors.sdsu.edu).

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HPLC Phytoplankton Pigment Measurements: Continuation of Climate Quality Data Records (CQDR) in Support of NASA’s Earth

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  1. HPLC Phytoplankton Pigment Measurements: Continuation of Climate Quality Data Records (CQDR) in Support of NASA’s Earth Science Enterprise (ESE) C.C. Trees and J.L. Mueller Center for Hydro-Optics and Remote Sensing (CHORS) San Diego State University (ctrees@chors.sdsu.edu) MODIS Science Team Meeting (BWI, MD, 22-24 Mar 05)

  2. HPLC Phytoplankton Pigment Protocols (Bidigare et al., 2003; NASA/TM-2003-211621/Rev 5 - Vol. V) • MODIS Data Products Program ~4,800 samples/yr Method Comparison Co-elution of mono- and divinyl Chl a 1. Wright et al. (1991) C18 Method (SIMBIOS) Channels ratio (436 & 450nm) • Physical separation of mono- and divinyl chl a • 1.Goericke and Repeta (1993) C8 Method • 2. **Van Heukelem and Thomas (2001) C8 with • column heater Method MODIS Science Team Meeting (BWI, MD, 22-24 Mar 05)

  3. Divinyl & Monovinyl Chl a(664 nm) Divinyl and Monovinyl Chl a Separation Divinyl Chl a Monovinyl Chl a Divinyl Chl b MODIS Science Team Meeting (BWI, MD, 22-24 Mar 05)

  4. Culture Mixture (450 nm) Mixture of Pigments from Cultures Fucoxanthin Monovinyl Chl a Chl c3 Injection Spike Diadinoxanthin 19’-Hexanoyloxyfucoxanthin Monovinyl Chl b Peridinin Chl c2 b.b carotene Zeaxanthin Prasinoxanthin Monovinyl Chls a’, a” Alloxanthin Diatoxanthin b.e carotene MODIS Science Team Meeting (BWI, MD, 22-24 Mar 05)

  5. HPLC Samples Processed/Submitted MODIS Science Team Meeting (BWI, MD, 22-24 Mar 05)

  6. CHORS Pigment Database Total Accessory = 1.082 Total Chl a 0.906 R2 = 0.956 n = 20,297 MODIS Science Team Meeting (BWI, MD, 22-24 Mar 05)

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