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Announcements

Announcements. Exam 1 is on Monday Multiple choice: 15-20 questions Matching: 8-10 questions Problems: 4-6 multipart problems (probably ~50% of total points) Multiple choice/matching problems from lecture, key terms/concepts, and homeworks (covers reading up through Chapter 5)

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Announcements

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  1. Announcements • Exam 1 is on Monday • Multiple choice: 15-20 questions • Matching: 8-10 questions • Problems: 4-6 multipart problems (probably ~50% of total points) • Multiple choice/matching problems from lecture, key terms/concepts, and homeworks (covers reading up through Chapter 5) • Problems similar (NOT IDENTICAL) to Homeworks 1-3 • Homework keys and term lists are all on the course website • Bring a #2 pencil! • You may use a calculator, but no phones allowed Corrected Homework 2 key posted to course website (error in Question #3c). Everybody gets 2.5 points! (up to 60 total)

  2. BIO 304 Lecture 10

  3. Interference

  4. Interference Recombination frequency v and cv: 19.6 Recombination frequency v and ct: 13.2 Recombination frequency cv and ct: 6.4 v ct cv 13.2 m.u. 6.4 m.u.

  5. Tetrad analysis

  6. Ordered tetrad analysis:mapping the centromere

  7. Example problem: A cross was made between two Neurospora strains: strain 1 was genotype A, strain 2 was genotype a. The following numbers of tetrads were produced: Tetrad Number AAaa 36 aaAA 44 AaAa 4 aAaA 6 AaaA 3 aAAa 7 What is the distance from the A gene to the centromere?

  8. Diagram the meiosis that produced the AAaa tetrad Diagram the meiosis that produced the AaaA tetrad

  9. An cross in Neurospora between a parent of genotype D and a parent of genotype d produced the following ordered tetrads. Which one(s) represent(s) first division segregation? 1. D D dd 2. D d D d 3. D dd D 4. dd D D 5. 1 and 3 6. 2 and 3 ✓ 7. 1 and 4

  10. Unordered tetrad analysis:linkage and mapping

  11. Example problem: A cross was made between two Chlamydomonas strains: strain 1 was genotype AB, strain 2 was genotype ab. The following numbers of tetrads were produced: Tetrad 1Tetrad 2Tetrad 3Tetrad 4Tetrad 5Tetrad 6 AB AB aBababab AB abaBaB AB Ab ab AB AbAbAbaB ababAb AB aB AB__ 38 5 6 17 2 3 1. Which tetrads represent the parental ditypes (PD), nonparentalditypes (NPD), and the tetratypes (TT)?

  12. Tetrad 1Tetrad 2Tetrad 3Tetrad 4Tetrad 5Tetrad 6 AB AB aBababab AB abaBaB AB Ab ab AB AbAbAbaB ababAb AB aB AB__ 38 5 6 17 2 3 2. Determine the map distance between genes A and B

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