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Announcements. Exam 1 is on Monday Multiple choice: 15-20 questions Matching: 8-10 questions Problems: 4-6 multipart problems (probably ~50% of total points) Multiple choice/matching problems from lecture, key terms/concepts, and homeworks (covers reading up through Chapter 5)

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announcements
Announcements
  • Exam 1 is on Monday
  • Multiple choice: 15-20 questions
  • Matching: 8-10 questions
  • Problems: 4-6 multipart problems (probably ~50% of total points)
  • Multiple choice/matching problems from lecture, key terms/concepts, and homeworks (covers reading up through Chapter 5)
  • Problems similar (NOT IDENTICAL) to Homeworks 1-3
  • Homework keys and term lists are all on the course website
  • Bring a #2 pencil!
  • You may use a calculator, but no phones allowed

Corrected Homework 2 key posted to course website (error in Question #3c). Everybody gets 2.5 points! (up to 60 total)

interference1
Interference

Recombination frequency v and cv: 19.6

Recombination frequency v and ct: 13.2

Recombination frequency cv and ct: 6.4

v

ct

cv

13.2 m.u.

6.4 m.u.

example problem
Example problem:

A cross was made between two Neurospora strains: strain 1 was genotype A, strain 2 was genotype a. The following numbers of tetrads were produced:

Tetrad Number

AAaa 36

aaAA 44

AaAa 4

aAaA 6

AaaA 3

aAAa 7

What is the distance from the A gene to the centromere?

slide8

Diagram the meiosis that produced the AAaa tetrad

Diagram the meiosis that produced the AaaA tetrad

slide9

An cross in Neurospora between a parent of genotype D and a parent of genotype d produced the following ordered tetrads. Which one(s) represent(s) first division segregation?

1. D D dd

2. D d D d

3. D dd D

4. dd D D

5. 1 and 3

6. 2 and 3

✓ 7. 1 and 4

example problem1
Example problem:

A cross was made between two Chlamydomonas strains: strain 1 was genotype AB, strain 2 was genotype ab. The following numbers of tetrads were produced:

Tetrad 1Tetrad 2Tetrad 3Tetrad 4Tetrad 5Tetrad 6

AB AB aBababab

AB abaBaB AB Ab

ab AB AbAbAbaB

ababAb AB aB AB__

38 5 6 17 2 3

1. Which tetrads represent the parental ditypes (PD), nonparentalditypes (NPD), and the tetratypes (TT)?

slide13

Tetrad 1Tetrad 2Tetrad 3Tetrad 4Tetrad 5Tetrad 6

AB AB aBababab

AB abaBaB AB Ab

ab AB AbAbAbaB

ababAb AB aB AB__

38 5 6 17 2 3

2. Determine the map distance between genes A and B