0. Genomic Southern Blotting and DNA Fingerprinting. ABE Workshop 2007 Jamie Lum. 0. Southern Blotting is a method to check for the presence of a gene or DNA in a sample. . 0.
1kB WT HM HZ LD 1kb WT HM HZ LD 1kB WT HM HZ LD 1kB WT HM HZ LD
Mark U C U C U C Mark C U C U C U Mark C U C U C U Mark U C U C U C
Immerse gel in 0.125M HCl and shake for 10 minutes.
Soak the gel in Denaturation Buffer for 30 minutes on shaker.
Rinse the gel with water.
Soak in Neutralization Buffer for 30 minutes.
Cut nylon membrane and two 3M filter papers to the size of the gel. Cut off top right corner of membrane.
Add transfer buffer to a glass tub.
LAYER, LAYER, AND LAYER!!
0Capillary transfer of the DNA fromthe gel to a nylon membrane
Mark gel wells on the membrane with a pencil.
Rinse the membrane in 6X SSC for 10 minutes.
Lay the membrane, nucleic acid side up, on a piece of foil and expose to 120,000 microjoules/cm2.
Someone ate the last cookie. A small DNA sample was taken from the plate and DNA was taken from seven of the workshop facilitators. Based on the RFLP, who did it?
CS-Crime Scene DNA
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A woman has accused a man of fathering her twins. Of course, he denies it, so a paternity test was done.
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