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Cell Counts by Hemocytometer

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# Cell Counts by Hemocytometer - PowerPoint PPT Presentation

Cell Counts by Hemocytometer. 9 large squares – 1mm x 1mm Center square divided into 25 smaller squares - 0.2mm x 0.2mm = 0.04 mm 2 Depth of the counting chamber – 0.1 mm Volume of a small square – 0.04 mm 2 x 0.1 mm = 0.004 mm 3 1 ml = 1 cm 3 = 1000 mm 3 (10 mm = 1 cm).

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## Cell Counts by Hemocytometer

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### Cell Counts by Hemocytometer

9 large squares – 1mm x 1mm
• Center square divided into 25 smaller squares - 0.2mm x 0.2mm = 0.04 mm2
• Depth of the counting chamber – 0.1 mm
• Volume of a small square – 0.04 mm2 x 0.1 mm = 0.004 mm3
• 1 ml = 1 cm3 = 1000 mm3 (10 mm = 1 cm)
Zoox Density Calculations
• Average of 10 counts of 0.004 mm3 = 6 zoox
• 0.004 mm3 x 1 ml/1000mm3 = 4 x 10-6 ml
• 6 zoox/4 x 10-6 ml = 1.5 x 106 zoox/ml
• Total homogenate volume = 5 ml
• Total zoox = 5ml x 1.5 x 106 zoox/ml = 7.5 x 106 zoox
• Wet weight of polyps = 0.25 g
• Zoox density:

7.5 x 106 zoox/0.25 g wet wt. = 3.0 x 107 zoox/g wet wt.