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Forensic Entomology (Part II). Maggot Crop Analysis. Maggot as a Source of Human DNA. Maggots store food in their crops as they feed. During later stages of larval development, the contents of the crop is emptied into the gut. CROP. Mouth. Posterior. For example:.

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maggot as a source of human dna
Maggot as a Source of Human DNA
  • Maggots store food in their crops as they feed.
  • During later stages of larval development, the contents of the crop is emptied into the gut

CROP

Mouth

Posterior

For example:

In Calliphora vicina at room temperature, the crop is full after 3 days and is emptied by 7 days (approximate)

slide5

Forensic DNA Review

DNA is found in the cells in our body.

Nucleus

(Brain of the cell)

Mitochondria

(more later)

slide6

Maggot Crop Analysis

Identification of maggot crop contents would be helpful in three situations:

Corpse is removed from suspected crime scene

slide7

Maggot Crop Analysis > Corpse Removed

A murder victim remains undiscovered.

Flies discover the corpse and lay eggs.

slide8

Maggot Crop Analysis > Corpse Removed

A murder victim remains undiscovered.

Flies discover the corpse and lay eggs.

slide9

Maggot Crop Analysis > Corpse Removed

Maggots hatch and feed on the corpse.

slide10

Maggot Crop Analysis > Corpse Removed

Murder victim is removed from crime scene.

The maggots remain.

slide11

Maggot Crop Analysis > Corpse Removed

Crop will contain Human tissue

slide12

Maggot Crop Analysis

Identification of maggot crop contents would be helpful in three situations:

There is a question as to the origin of a maggot used in a PMI estimation

slide13

Maggot Crop Analysis > Maggots from Other Source

A turkey is thrown into a dumpster.

slide14

Maggot Crop Analysis > Maggots from Other Source

A turkey is thrown into a dumpster.

slide15

Maggot Crop Analysis > Maggots from Other Source

Three days later, maggots have developed on the turkey.

slide16

Maggot Crop Analysis > Maggots from Other Source

Also three days later, a dead body is thrown into the dumpster.

slide17

Maggot Crop Analysis > Maggots from Other Source

The maggots migrate from the turkey to the human body.

slide18

Maggot Crop Analysis > Maggots from Other Source

The maggots migrate from the turkey to the human body.

slide19

Maggot Crop Analysis > Maggots from Other Source

Crop will contain Turkey tissue or a mixture of Turkey/Human tissue

slide20

Maggot Crop Analysis

Identification of maggot crop contents would be helpful in three situations:

There may be a chain of evidence dispute.

slide22

Maggot Crop Analysis > Chain of Evidence Dispute

2 days later...

They’re 5 days old!

These aren’t the

maggots I gave you.

Are too!

Are not!

slide23

Maggot Crop Analysis

Poor quality of samples usually prevents analysis.

maggot dissection
Maggot Dissection

Maggot before dissection

maggot dissection1
Maggot Dissection

Cut off posterior segments

maggot dissection2
Maggot Dissection

Ventral incision of the cuticle

maggot dissection3
Maggot Dissection

Peel back cuticle to reveal crop