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Immune Response Modulation in Uraemic Monocytes: CD Markers and Thymidine Incorporation Analysis

This study analyzes the surface expression of CD40, CD80, and CD86 on freshly isolated and LPS-stimulated uraemic and normal monocytes under different culture conditions, including complete and uraemic media. Results demonstrate significant differences in Mean Fluorescence Intensity (MFI) values, indicating altered immune responses in uraemic monocytes. Thymidine incorporation assays reveal impaired proliferation in uraemic monocytes. Statistical significance was confirmed using the Mann-Whitney U test, highlighting the effects of uraemia on monocyte functionality and their downstream effects on dendritic cells.

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Immune Response Modulation in Uraemic Monocytes: CD Markers and Thymidine Incorporation Analysis

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  1. (i) Freshly isolated uraemic monocytes CD40* CD80* CD86* MFI 1.4±0.4 MFI 1.2±0.3 MFI 1.3±0.5 (ii) LPS-stimulated uraemic monocytes in complete medium MFI 3.3±1.1 MFI 1.9±0.5 MFI 2.2±0.7 (iii) LPS-stimulated uraemic monocytes in autologous uraemic medium MFI 0.0±0.0 MFI 0.0±0.0 MFI 0.0±0.0 (iv) LPS-stimulated normal monocytes in complete medium MFI 8.7±1.6 MFI 2.7±0.6 MFI 3.3±0.9

  2. [3H] thymidine incorporation (cpm) *p<0.0001 Monocyte numbers Results expressed as mean±SD, Mann Whitney U *p<0.0001

  3. LPS-stimulated normal monocyte-derived DC in complete medium CD14 CD40 CD80 CD83 CD86* MFI 0.2±0.2 MFI 104.2±54.3 MFI 7.5±3.6 MFI 3.7±2.8 MFI 23.1±4.3 LPS-stimulated normalmonocyte-derived DC cultured in uraemic medium (CAPD) MFI 0.2±0.1 MFI 111.5±24.6 MFI 3.2±2.6 MFI 0.8±0.5 MFI 11.1±4.7 LPS-stimulated normalmonocyte-derived DC cultured in uraemic medium (CRF) MFI 0.8±0.2 MFI 110.2±64.3 MFI 4.3±2.7 MFI 1.4±0.9 MFI 15.2±7.2

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