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Protein delivery: DNA nanostructures and cell-surface targeting

Protein delivery: DNA nanostructures and cell-surface targeting. Harvard iGEM August 27, 2006. The Machine. Goal: Future modularized drug delivery. target cell. protein. DNA Nanostructures Overview. Can design DNA double helices to stick together and form interesting structures. .

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Protein delivery: DNA nanostructures and cell-surface targeting

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  1. Protein delivery:DNA nanostructures and cell-surface targeting Harvard iGEM August 27, 2006

  2. The Machine • Goal: Future modularized drug delivery target cell protein

  3. DNA Nanostructures Overview • Can design DNA double helices to stick together and form interesting structures. • Dr. Ned Seeman, NYU • Dr. William Shih, Harvard • Paul Rothemund, Caltech A 1.7-kilobase single-stranded DNA that folds into a nanoscale octahedron WILLIAM M. SHIH, JOEL D. QUISPE & GERALD F. JOYCE
Nature427, 618ﾐ621 (2004); doi:10.1038/nature02307

  4. Motivation: Why DNA? • Fascinating area of research • The power of DNA • Watson-Crick base pairing is enormously strong • Self-assembly • Highly programmable, designable • Specificity - targeting to cells

  5. Design Details

  6. Design Details: Scaffolded Oragami

  7. Design Details: Scaffolded Oragami

  8. Design Details: Scaffolded Oragami

  9. Design Details: Positional Control

  10. Design Details: Positional Control

  11. Design Details: Positional Control

  12. Design Details: Positional Control

  13. Design Details: Positional Control

  14. Design Details: Positional Control

  15. Progress • Built a number of barrel designs • Exciting EM Images • Purifying Nanostructures (nearly there after 1 month of trials)

  16. Exciting EM Images

  17. Exciting EM Images

  18. EM Images (snakes on a grid) • c5.0 barrel (10 nM), 0.7% uranyl formate • Appear to be lining up end to end, probably because of the stain Images courtesy Shawn Douglas

  19. To be continued • Can a protein be protected from protease if attached inside the box? • Lid attachment • Lid removal protease protein protease protein

  20. Acknowledgements • Harvard TFs -Shawn Douglas, Nick Stroustrup, Chris Doucette • Harvard advisers- Dr. William Shih, Dr. George Church, Dr. Pamela Silver, Dr. Alain Viel, Dr. Jagesh Shah, Dr. Radhika Nagpal • iGEM ambassadors • iGEM directors

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