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Laboratory Report on Heritability in Subclinical Disease Study

This laboratory report details ongoing assays in the MESA Family study to investigate heritability in relation to subclinical disease, supporting candidate gene research. Completed assays include lipids, glucose, creatinine, multiplex panels for adipokines, IL-6, CRP, D-dimer, and PAI-1. Data on probands and siblings in MESA Classic and De Novo families are analyzed, and technical error of measurement is calculated. Tasks include resolving QC discrepancies, addressing assay issues, and completing remaining assays on MESA Family participants.

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Laboratory Report on Heritability in Subclinical Disease Study

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  1. Laboratory Report

  2. MESA Family Assays • To study heritability in relation to subclinical disease • To support candidate gene work

  3. Ongoing assays: Lipids (total, HDL and LDL cholesterol and triglycerides) Glucose Creatinine

  4. Other assays: • Multiplex panels for adipokines: • Adiponectin, resistin, leptin, TNF-α, MCP-1 • Insulin* • IL-6* • CRP* • D-dimer* • PAI-1** • *Measured in full MESA cohort • **Measured in MESA 1000-person sample (Group 3)

  5. Assay Progress • Completed on first ½ of MESA Family Participants (n=666) • IL-6 • Insulin (also MESA Classic participants using new method)* • CRP* • D-dimer* • *data released

  6. * Lab Variables by Family Type *Probands and siblings in MESA Classic Classic families: proband in MESA Classic, siblings recruited into MESA Family De Novo Families: entirely recruited outside of MESA page 21 of Steering Committee Report

  7. Lab Variables in MESA and MESA Family page 23 of Steering Committee Report

  8. Lab Variables in MESA and MESA Family page 23 of Steering Committee Report

  9. Lab Variables in MESA and MESA Family page 23 of Steering Committee Report

  10. Percent technical error of measurement - TEM% *Calculated value: LDL=total cholesterol - HDL - (triglycerides/5) x’s = paired values and y’s = QC values with x, y as measurements from the same individual; measurements on n participants

  11. HDL – MESA Family Value versus QC Value

  12. Glucose – MESA Family Value versus QC Value

  13. Tasks • Resolve QC discrepancies • Resolve IL-6 assay issue • Remaining assay work • Insulin, IL-6, CRP and D-dimer assays on second ½ of MESA Family participants • Adipokine panels – will be run on full MESA Family cohort including MESA Classic participants • PAI-1 – will be run on full MESA Family cohort including MESA Classic participants

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