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Investigating Cell Cycle Distribution and Protein Expression in BRCA1/BARD1 Post Serum Starvation

This study analyzes the effects of serum starvation on cell cycle distribution and the expression of key proteins such as BRCA1, BARD1, Skp2, FBXO44, and a-Tubulin in HEK293T cells. We utilize a range of techniques, including SiRNA assays, to observe changes in protein levels and cell cycle phases. The results illustrate how serum deprivation influences the regulatory mechanisms of the cell cycle, providing insights into potential implications for cancer research and therapeutic strategies.

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Investigating Cell Cycle Distribution and Protein Expression in BRCA1/BARD1 Post Serum Starvation

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  1. A. B. Asyn 0 h 12 h 16 h 20 h 24 h 26 h 28 h 36 h Release post serum starvation BRCA1 BARD1 Cell Cycle Distribution (%) Skp2 FBXO44 Skp1 a-Tubulin Release from serum starvation T24 C. 52.87 51.35 48.98 39.98 37.99 37.38 33.5 32.48 26.52 Cell Cycle Distribution (%) 16.17 13.63 9.13 SiRNA HEK293T

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