Nanomaterial’s Application in Biotechnology. Lei Sun Project manager Digital Health Group Intel Corp. Outline. Overview of nanomaterial’s application in biotech, especially in cancer diagnosis and treatment. Application Example I: Gold Nanoparticle’s application in DNA detection.
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Digital Health Group
PSA (prostate specific antigen)
HER-2 (Breast cancer biomarker)
CA-125 (Ovarian cancer biomarker)
At this stage, cancel cells have to detected through ultra-sensitive biomarkers
TEM image of the cluster of DNA-functionalized gold nanoparticles
DNA probe Sequences are designed so that the target DNA will link the particles together
Color change of the gold colloidal solution reflect change of the cluster size
The gold nanoparticles are linked together by target DNA
Measure the resistance between 2 electrodes to tell the concentration of target DNA
Sensitivity 500 fmol or 109 molecules.
Virus detection – HIV, SARS, Bird flu, etc.
Biomarker detection – DNA/RNA related to cancer and drug metabolism.
Personalized Medicine – Predict each patient’s response to certain treatment based on his/her gene.
Highly fluorescent, nanometer-size, single crystals of semiconductor materials
655605585565 525 nm
Size of the nanocrystal determines the color
Size is tunable from ~5-15 nm (±3%)
Size distribution determines the spectral width
Core Nanocrystal (CdSe)
- Determines color
Inorganic Shell (ZnS)
– Improves brightness and stability
- Provides water solubility and functional groups for conjugation.
Minimal (<5%) cross-talk using 20nm bandpass filters
QdotExample Application - Qtracker® Cell Labeling Kits
Goal of Qtracker:
To provide customers an approach to track live cells using Qdot-sav.
No Biotin 10x Biotin 50X Biotin
As the concentration of quantum dots increases, the signal strength from the cell increases
The cells are still alive after multiple days. When the cells divide, the dots in the cell are divided also, causing signal to drop according to division rate.
Different Qdots can be functionalized so that each dot can enter a specific type of cells. Enables multi-color labeling and tracking. Cell-cell interaction can be studied with the same approach.
Cell’s migration speed can be measured by scratch an “X” mark in a cell culture, then monitor the time it takes for the cells to cross the boundary.