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Input. Pull down. MBP-PIF4. MBP. pulled down by GST-PAR1. Supplementary Figure 1. In vitro pull-down assay shows specific interaction between PAR1 and PIF4.

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slide1

Input

Pull down

MBP-PIF4

MBP

pulled down by GST-PAR1

Supplementary Figure 1. In vitro pull-down assay shows specific interaction between PAR1 and PIF4.

MBP or MBP-PIF4 was pulled down by GST-PAR1 immobilized on glutathione beads. Proteins bound to GST-PAR1 were detected by immunoblotting using anti-MBP antibody. Only MBP-PIF4, but not MBP alone, was pullded down by GST-PAR1 suggesting specific interaction between PAR1 and PIF4.