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fibroblast Division in Microgravity. Jennifer Jiang, Jasmine Kuo , Kara Lukas San Marino High School San Marino, California. Background. Cell division is an integral component of life. All organisms must go through the cell cycle in order to grow, develop, and reproduce.

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fibroblast division in microgravity

fibroblast Division in Microgravity

Jennifer Jiang, Jasmine Kuo, Kara Lukas

San Marino High School

San Marino, California

background
Background
  • Cell division is an integral component of life.
  • All organisms must go through the cell cycle in order to grow, develop, and reproduce.
  • Past experiments in simulated microgravity have shown that lack of gravity causes cells to divide at a slower rate.
purpose
Purpose
  • To discover the effect of microgravity on cell division
  • To compare the rates of cell division in the absence and presence of gravity
hypothesis
hypothesis
  • If cells are exposed to microgravity during their life cycles, then there will be a reduction in cell division because of
    • disorganization of microtubules
    • change in actin filaments
    • chromatin less dense
    • increased apoptosis
materials
Materials
  • Type 3 FME
  • Mouse embryonic fibroblasts
  • Eagle’sMinimum Essential Medium (EMEM)
  • FetalBovine Serum (FBS)
  • Formaldehyde
  • Light Microscope
  • Hemocytometer
  • Trypan Blue
assembly procedures
Assembly procedures

Place experiment samples into the appropriate compartment of the FME.

  • Main Volume
    • 5.6 mL of EMEM
    • 0.56mL of 100% FBS
  • Short Ampoule A
    • 0.92 ml fibroblast cells
  • Short Ampoule B
    • 0.92 ml formaldehyde
experiment
experiment
  • Initiation: crack Ampoule A
  • Termination: crack Ampoule B
  • Experiment lasted 9 days
slide8
Data

Number of fibroblasts

analysis conclusion
Analysis/Conclusion
  • Growth is more favorable for cells in 37°C CO2 incubator.
  • Cell growth is greater when the original cell count is increased from 500 to 750.
  • More conclusions will be drawn after flight experiment returns.
acknowledgements
Acknowledgements
  • Mr. Wyeth Collo of San Marino High School
  • Dr. Susan Kane and Ms. Erin Denny of City of Hope Cancer Center
  • Center for the Advancement of Science in Space
  • San Marino Unified School District PTSA
  • Stephen and Mary Birch Foundation
  • Smithsonian Air and Space Museum
  • NanoRacks
  • Student Spaceflight Experiments Program