fibroblast division in microgravity n.
Skip this Video
Loading SlideShow in 5 Seconds..
fibroblast Division in Microgravity PowerPoint Presentation
Download Presentation
fibroblast Division in Microgravity

Loading in 2 Seconds...

play fullscreen
1 / 10

fibroblast Division in Microgravity - PowerPoint PPT Presentation

  • Uploaded on

fibroblast Division in Microgravity. Jennifer Jiang, Jasmine Kuo , Kara Lukas San Marino High School San Marino, California. Background. Cell division is an integral component of life. All organisms must go through the cell cycle in order to grow, develop, and reproduce.

I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
Download Presentation

PowerPoint Slideshow about 'fibroblast Division in Microgravity' - vicki

An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.

- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript
fibroblast division in microgravity

fibroblast Division in Microgravity

Jennifer Jiang, Jasmine Kuo, Kara Lukas

San Marino High School

San Marino, California

  • Cell division is an integral component of life.
  • All organisms must go through the cell cycle in order to grow, develop, and reproduce.
  • Past experiments in simulated microgravity have shown that lack of gravity causes cells to divide at a slower rate.
  • To discover the effect of microgravity on cell division
  • To compare the rates of cell division in the absence and presence of gravity
  • If cells are exposed to microgravity during their life cycles, then there will be a reduction in cell division because of
    • disorganization of microtubules
    • change in actin filaments
    • chromatin less dense
    • increased apoptosis
  • Type 3 FME
  • Mouse embryonic fibroblasts
  • Eagle’sMinimum Essential Medium (EMEM)
  • FetalBovine Serum (FBS)
  • Formaldehyde
  • Light Microscope
  • Hemocytometer
  • Trypan Blue
assembly procedures
Assembly procedures

Place experiment samples into the appropriate compartment of the FME.

  • Main Volume
    • 5.6 mL of EMEM
    • 0.56mL of 100% FBS
  • Short Ampoule A
    • 0.92 ml fibroblast cells
  • Short Ampoule B
    • 0.92 ml formaldehyde
  • Initiation: crack Ampoule A
  • Termination: crack Ampoule B
  • Experiment lasted 9 days

Number of fibroblasts

analysis conclusion
  • Growth is more favorable for cells in 37°C CO2 incubator.
  • Cell growth is greater when the original cell count is increased from 500 to 750.
  • More conclusions will be drawn after flight experiment returns.
  • Mr. Wyeth Collo of San Marino High School
  • Dr. Susan Kane and Ms. Erin Denny of City of Hope Cancer Center
  • Center for the Advancement of Science in Space
  • San Marino Unified School District PTSA
  • Stephen and Mary Birch Foundation
  • Smithsonian Air and Space Museum
  • NanoRacks
  • Student Spaceflight Experiments Program