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Synechococcus PCC7942

Synechococcus PCC7942. Prochlorococcus MED. 100. Prochlorococcus SS120. 94. CC9311. 100. CC9617. WH7803. 0.4. 100. WH7805. No PUB. CC9318. 95. CC9317. 74. CC9305-3. PCC6307. No PE. 100. E. lake isolate. No PUB. CC9616. CC9701. 100. SS9401. 0.1 substitutions/site. 78.

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Synechococcus PCC7942

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  1. Synechococcus PCC7942 Prochlorococcus MED 100 Prochlorococcus SS120 94 CC9311 100 CC9617 WH7803 0.4 100 WH7805 No PUB CC9318 95 CC9317 74 CC9305-3 PCC6307 No PE 100 E. lake isolate No PUB CC9616 CC9701 100 SS9401 0.1 substitutions/site 78 CC9605 CC9703 0.4 0.9 92 C129 100 2.0 1.2 0.8 1.1 2.0 WH8102 WH8103 WH8011 WH8112 CC9301 100 Motile isolates WH8113 CC9702 1.0 1.6 Fig. 1 Neighbour-joining tree constructed using Jukes-Cantor corrected distances using a 612-bp fragment of the DNA- dependent RNA polymerase (rpoC1) gene sequence of different Synechococcus-like isolates. The values in boxes are PUB/PEB fluorescence ratios. (From Toledo et al., 1999).

  2. Prochlorococcus-specific 16S rDNA oligonucleotides Probe 645 HLI 3´-CTA GCC GAA CTC ATA CCA-5´ Target (E.coli 645-662) 5´-GAU CGG CUU GAG UAU GGU-3´ PCC9511/EQPAC1 (HLI) ... ... ... ... ... ... ENATL1 env.clone (HLI) ... ... ... ... ... ... ENATL4 env.clone (LL) ... U.. ... ... ... ... NATL1-MIT (LL) ... U.. ... ... ... ... TAK9803-2 (HLII) ..A A.. ... ... ... ... MIT9303/MIT9313 (LL)... U.. ... ... .G. ... SS120 (LL) ..A G.. ...... ... ... Syn.WH7803/WH8103 .U. G.. ... ... .G. ... Syn.WH8018 .U. G.. ..C ... .G. ... Syn.WH7805 .U. G.. .NC ... .G. ... Probe 645 HLII 3´-CTT TCC GAA CTC ATA CCA-5´ Target (E.coli 645-662) 5´-GAA AGG CUU GAG UAU GGU-3´ TAK9803-2 ... ... ... ... ... ... PCC9511/EQPAC1 ..U C.. ... ... ... ... ENATL1 env.clone ..U C.. ... ... ... ... ENATL4 env.clone ..U U.. ... ... ... ... NATL1-MIT ..U U.. ... ... ... ... MIT9303/MIT9313 ... U.. ... ... .G. ... SS120 ...G.. ... ... ... ... Syn.WH7803/WH8103.UU G.. ... ... .G. ... Syn.WH8018.UU G.. ..C ... .G. ... Syn.WH7805.UU G.. .NC ... .G. ... Probe 645 LL 3´-CTA ACC GAA CTC ATA CCA-5´ Target (E.coli 645-662) 5´-GAU UGG CUU GAG UAU GGU-3´ NATL1-MIT ... ... ... ... ... ... ENATL4 env.clone ... ... ... ... ... ... MIT9303/MIT9313 ... ... ... ... .G. ... TAK9803-2 ..A A.. ... ... ... ... PCC9511/EQPAC1 ... C.. ... ... ... ... ENATL1 env.clone ... C.. ... ... ... ... SS120 ..A G.. ... ... ...... Syn.WH7803/WH8103.U. G.. ... ... .G. ... Syn.WH8018.U. G.. ..C ... .G. ... Syn.WH7805.U. G.. .NC ... .G. ...

  3. Prochlorococcus-specific 16S rDNA oligonucleotides 181 HLI Prochlorococcus-specific probe Probe 181HLI 3´-TAC GGC TAT CCA CTT TAC-5’ Target(E.coli 181-198) 5´-AUG CCG ATA GGU GAA AUG-3´ PCC9511/EQPAC1 ... ... ... ... ... ... ENATL1 env.clone ... ... ... ... ... ... ENATL4 env.clone ... ... .G. ... ... ... NATL1-MIT/MIT9303/MIT9313 ... ... .G. ... ... ... SS120 ... ... .G. ... ... ... TAK9803-2 ... ..U .CU ... ... ... Syn sp.WH7805/7803/8018... ... .G. ... ... ... Syn sp.WH8103... ... CG. ... ... … 181 LL Prochlorococcus-specific probe Probe 181LL 3´-TAC GGC TCT CCA CTT TAC-5´ Target(E.coli 181-198) 5´-AUG CCG AGA GGU GAA AUG-3´ NATL1-MIT... ... ... ... ... ... MIT9303/MIT9313 ... ... ... ... ... ... SS120 ... ... ... ... ... ... ENATL4 env.clone ... ... ... ... ... ... Syn. sp.PCC6307 ... ... ... ... ... ... Syn.sp.WH7805/7803/8018 ...... ... ... ... ... Syn.sp. Biwa-B0/-G0/-P0 ... ... ... ... ... ... Oscillatoria neglecta M-82... ... ... ... ... ... Syn.sp. WH8103 ... ... C.. ... ... ... PCC9511/EQPAC1 ... ... .U. ... ... ... ENATL1 env.clone ... ... .U. ... ... ... TAK9803-2 ... ..U .CU ... ... …

  4. Prochlorococcus MIT9303/MIT9313/MIT9211 LL specific probe Probe 148LLMIT 3´-CTA TTG CCA ACC TTT GCC-5’ Target(E.coli 148-166) 5´-GAU AAC GGU UGG AAA CGG-3´ MIT9211/MIT9303/MIT9313 ... ... ... ... ... ... PCC9511/EQPAC1 ... ... ... ... ... ..A MIT9107/MIT9302/MIT9312 ... ... ... ... ... ..A SS120 ... ... ... ... ... ..A TAK9803-2 ... ... ... ... ... ..A Syn sp.WH8103 ... ... ... ... ... ..A Syn sp.WH7805 ... ... ..C ... ... ... ‘GENERAL’ Prochlorococcus probe Probe 405PRO 3´-ACT CCC TGC TTC CGG AGA-5´ Target(E.coli 405-422) 5´-UGA GGG ACG AAG GCC UCU-3´ PCC9511/EQPAC1 ... ... ... ... ... ... NATL1-MIT ... ... ... ... ... ... TAK9803-2... ... ... ... ... ... SS120 ... ... ... ... ... ... ENATL1 env.clone ... ... ... ... ... ... ENATL4 env.clone ... ... ... ... ... ... MIT9303/MIT9313 ... ... .U. ... ... ... Syn.sp. WH7803/7805/8103 ... ... .U. ... ... ...

  5. Phylogenetic relationships among marine Prochlorococcus & Synechococcus strains

  6. Phylogenetic relationships among marine Synechococcus strains

  7. Phylogenetic relationships among marine Synechococcus strains

  8. Comparison of gene clusters coding for phycobiliproteins Synechococcus WH 8020 14891 bp orfs 200,236,257 orf 196 mpeB A C U mpeV rpcB A rpcE F cpeA B cpeZ Y orf140 Prochlorococcusmarinus SS120 10153 bp Y cpeB A Z mpeX ppeC metK uvrD orf431 Orf195,181 Prochlorococcusmarinus MED4 2850 bp cpeB uvrD metK Orf181

  9. Phylogenetic relationships amongst Prochlorococcus strains Prochlorococcus sp. HL1SB3 (RCC283) Prochlorococcus sp. MED4 (RCC153) Prochlorococcus sp. EQPAC1 (RCC278) HLI Prochlorococcus sp. HL1GP2 (RCC281) Prochlorococcus sp. TATL4 Prochlorococcus sp. PCC9511 Prochlorococcus sp. EQPAC2 (RCC279) Prochlorococcus sp. TATL1A Prochlorococcus sp. NATL2 SAR6 Prochlorococcus sp. SB Prochlorococcus sp. TATL2 Prochlorococcus sp. MIT9302 HLII Prochlorococcus sp. MIT9312 Prochlorococcus sp. GP2 Prochlorococcus sp. TATL1B Prochlorococcus sp. MIT9107 Prochlorococcus sp. TAK9803 (RCC264) Prochlorococcus sp. NATL2A Prochlorococcus sp. MITNATL2 (RCC280) Prochlorococcus sp. PAC1 LL Prochlorococcus sp. MITNATL1 (RCC277) Prochlorococcus sp. SS120 (RCC156) SAR7 Prochlorococcus sp. MIT9303 Prochlorococcus sp. MIT9313 SAR139 SAR100 Synechococcus WH8103 Synechococcus WH7805 Synechococcus WH8101 Synechococcus PCC6307 Microcystis elabens Synechococcus PCC 6301 Oscillatoria corallinae 0.1

  10. Fluorescent in situ hybridisation Oligonucleotide Monolabeled-fluorochrome Fluorescein, Rhodamine, CY3, BODIPY C6 amino-link PROBE LABELING HRP-labeled Samples on filters or on glass slides 46 °C 35 °C Hybridisation HYBRIDISATION 48 °C 35 °C Washing Equilibriation in buffer Incubation with fluorochrome-tyramide Washing Observe under fluorescence microscope

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