Isolation of Rare Circulating Tumor Cells - PowerPoint PPT Presentation

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Isolation of Rare Circulating Tumor Cells

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  1. Isolation of Rare Circulating Tumor Cells Sunitha Nagrath et al. • Presentation by: • KearaDarragh, Matthew Faw, and Will Plautz http://www.nibib.nih.gov/news-events/newsroom/circulating-tumor-cells-captured-last

  2. Overview of Metastasis and CTCs • Circulating tumor cells (CTCs) • Likely the origin of intractable metastatic disease • 1 CTC : 10^9 haematologic cells in the blood of cancer patients

  3. CTC chip better than existing methods • Immunomagnetic-bead • purification Circulating Tumor Cell (CTC) Chip • First microfluidic device that can successfully isolate viable cells frommilliliter volumes of whole blood • High sensitivity, selectivity, and yield • Single step • Gross prognostic tool (high/low risk) • Isolates fewerCTCs with very low purity (<1%)and yield • Multiple bulk semi-automatedpreparatory steps

  4. What isMicrofluidics? • Deals with small volumes(fL, pL, nL, µL)of fluids • Generally processed in a way that moves, mixes, or separates the contents • Microfluids act differently • Surface tension and low Reynolds numberbecome larger factors in the system • Different types of microfluidics • Dealing with continuous-flow today

  5. What isMicrofluidics? • Deals with small volumes(fL, pL, nL, µL)of fluids • Generally processed in a way that moves, mixes, or separates the contents • Microfluids act differently • Surface tension and low Reynolds numberbecome larger factors in the system • Different types of microfluidics • Dealing with continuous-flow today

  6. What isMicrofluidics? • Deals with small volumes(fL, pL, nL, µL)of fluids • Generally processed in a way that moves, mixes, or separates the contents • Microfluids act differently • Surface tension and low Reynolds numberbecome larger factors in the system • Different types of microfluidics • Dealing with continuous-flow today

  7. How Does the Chip Capture Circulating Tumor Cells? • 25 mm x 66 mm silicon chip • 78,000 microposts • epCAM antibodies

  8. CTC Isolating Microfluidic Device 25mm

  9. Staining to Assess Purity • Stains are used in order to identify malignant cells from healthy cells. • Malignant cells look different • Large cellular size • Relatively large nuclei • Visible nucleoli

  10. Results and Applications • Successfully identified CTCs in 115/116 samples • Approximately 50% purity • CTCs isolated (per ml): • NSCLC: 5-1,281 • Breast: 5-174 • Monitor cancer treatment response

  11. Results and Applications • Successfully identified CTCs in 115/116 samples • Approximately 50% purity • CTCs isolated (per ml): • NSCLC: 5-1,281 • Breast: 5-174 • Monitor cancer treatment response

  12. Conclusion • First microfluidic device that can successfully process milliliter volumes of whole blood • High sensitivity, selectivity, and yield • Single step • Isolation of viable cells • Allows for real time monitoring of cancer progression in a non-invasive manner