chapter 16 mutation repair and recombination
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Chapter 16 Mutation, Repair and Recombination

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slide4
Question
  • Lysine and arginine are two amino acids with similar sized “R” (side-) groups and similar biochemical behavior (for example, both are basic residues). A mutation results in substitution of lysine for arginine and has no detectable effect on the function of that protein. Which statement best applies to this situation?
  • The mutation is a silent mutation.
  • The mutation is a frameshift mutation.
  • The amino acid change is conservative.
  • The amino acid change is silent.
  • This organism displays no codon bias.
slide6
Question
  • Which of the following represents a correct or acceptable phrase in genetics?
  • Mutant protein
  • Protein mutation
  • DNA mutation
  • A and C
  • All of the above
slide10
Question
  • Which of the following statements is correct about the bases in DNA under normal circumstances:
  • Adenine should pair with thymine.
  • The bonds holding guanine and cytosine together are stronger than the bonds holding adenine and thymine together.
  • A purine always binds to a pyrimidine.
  • A substitution of adenine for guanine is a transition mutation.
  • All of the above
slide24
Repair mechanisms
  • Photo reactivation repair
  • Base excision repair
  • Nucleotide excision repair
  • Transcription coupled repair
  • Mismatch repair
slide27
Undamaged DNA

UV light (mutagenic agent)

EMS, NG (mutagenicagent)

Pyrimidine dimers

Methylated base

Visible light

Inactivated after action

Alkyl Transferase

Photolyase

Original Base

slide37
Question
  • Which of the following repair pathways do not involve the use of a homology-dependent repair system?
    • Base excision repair
    • Photo reactivation repair
    • Transcription coupled repair
    • Nucleotide excision repair
    • Mismatch repair
slide38
Question
  • Which of the following DNA duplexes is most stable?
    • 5’AATTAATTAATTAATTAATT 3’
  • 3’TTAATTAATTAATTAATTAA 5’
  • 5’ GGCCGGCCGGCCGGCCGGCC 3’
  • 3’ CCGGCCGGCCGGCCGGCCGG 5’
    • Both are equally stable.
    • B is more stable than A.
slide39
Question
  • Which of the following repair pathways do not involve the use of a homology-dependent repair system?
    • Base excision repair
    • Photo reactivation repair
    • Transcription coupled repair
    • Nucleotide excision repair
    • Mismatch repair
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