1 / 67

DNA - The Code of Life

DNA - The Code of Life. Chapter 16. Bacterial Transformation. F. Griffith & Transformation. 1928 Streptococcus pneumoniae 2 strains: R – harmless, S – pathogenic Mixed inactive S strain & active R strain; injected into mouse Mouse died, pathogenic strain in blood Transformation.

verne
Download Presentation

DNA - The Code of Life

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. DNA - The Code of Life Chapter 16

  2. Bacterial Transformation

  3. F. Griffith & Transformation • 1928 • Streptococcus pneumoniae • 2 strains: R – harmless, S – pathogenic • Mixed inactive S strain & active R strain; injected into mouse • Mouse died, pathogenic strain in blood • Transformation

  4. Avery, McCarty & MacLeod • 1944 : transforming agent is DNA • Skeptics – bacteria not complex • More research • Viruses (bacteriophages) • Viral structure & replication?

  5. y

  6. Hershey & Chase – The Blender Experiment • 1952: DNA is the genetic material of the phage T2 • T2 phage infects E. coli • Labeled protein coat with radioactive S and the DNA with radioactive P • Phages infect E. coli separately • Only P found in the bacterium

  7. Findings • Infected with radio-labeled proteins - radioactivity in supernatant • Infected with radio-labeled DNA - radioactivity in pellet • Hershey & Chase’s conclusion?

  8. Let the race begin! • 1950s – scientific community racing to find 3-D structure of DNA • Major players • James Watson & Francis Crick • Linus Pauling • Maurice Wilkins & Rosalind Franklin

  9. Puzzle Pieces • Chargaff’s Data • Backbone Structure – single strand • Franklin’s Data

  10. Chargaff’s Rules - Structure • 1947 • Polymer - deoxyribose sugar, phosphate grp & nitrogen-containing base • Bases: • adenine (A), thymine (T), guanine (G), or cytosine (C)

  11. Chargaff’s Rules • Certain bases were always equal in number • # adenines approx equal to # of thymines (%T = %A) • # guanines approx equal to # of cytosines (%G = %C) • Why is this significant?

  12. Backbone Structure • Phosphate group of one nucleotide attached to the sugar of the next • Result is a “backbone” of phosphates and sugars, from which the bases project

  13. R. Franklin & M. Wilkins X-Ray Crystallography

  14. Franklin’s Data • Wilkins & Franklin used X-ray crystallography to study DNA structure • X-rays diffracted as they pass through aligned fibers of purified DNA • Diffraction pattern used to deduce 3-D shape of molecules

  15. What the picture tells us • DNA was helical in shape!

  16. Putting the puzzle together… • Watson & Crick – “stolen” ideas or a product of the times? • However… it was Watson who deduced the width of the helixand the spacing of bases • Model building to beat Pauling

  17. TRIAL AND ERROR • Watson & Crick began to work on a model of DNA with two strands, the double helix • Wire molecular models - first tried to place the sugar-phosphate chains on the inside • Did not fit the X-ray measurements and other info on chemistry of DNA

  18. Breakthrough • Watson put sugar-phosphate chain on the outside & nitrogen bases on the inside

  19. Nuts & Bolts – Chargaff’s Data • Watson & Crick determined that chemical side groups off nitrogen bases formed H bonds, connecting strands • Adenine - form 2 H bonds only with thymine • Guanine - form 3 H bonds only with cytosine • Findings explained Chargaff’s rules

  20. DNA Structure • DNA bases: • Purines: adenine and guanine • Pyrimidines: thymine and cytosine • Nucleotides are covalently bonded with a sugar-phosphate backbone • The linkage forms a 3’,5’ phosphodiester linkage • One end of the molecule has a free 5’ carbon; the other has a free 3’ carbon

  21. DNA Structure • Two polynucleotide chains intertwined to form a double helix

  22. Technical DNA Data • .34 nm is the distance between the bases • 3.4 nm repeat of nucleotides due to a complete “turn” of the helix • width of molecule is 2.0 nm

  23. Pyrimidines and Purines • Pyrimidines are single-ringed • Purines are double-ringed • Bonding is complementary • Sequence in one chain dictates sequence in opposite chain

  24. DNA Replication • Complimentary bases act as templates • Bases of one strand allow for exact duplication

  25. DNA Replication • Origin(s) of replication • Prokaryotes - single specific sequence of nucleotides recognized by replic. enzymes • Replication proceeds in both directions • Eukaryotes – hundreds/thousands of origin sites per chrom • Bubble with replication forks at each end • Bubbles elongate as DNA is replicated and eventually fuse

  26. Bidirectional Synthesis • In prokaryotes, the circular DNA is opened up, and synthesis occurs in both directions

  27. Bidirectional Synthesis • In eukaryotes, the linear DNA has many replication forks

  28. DNA Replication • Proteins and enzymes work together • DNA strands must be unwound during replication • DNA helicase unwinds the strands • Single stranded binding proteins (SSB) prevent immediate reformation of the double helix • Topoisomerases break and then rejoin the strands, “untying” the knots that form

  29. DNA Replication Order • Always proceeds in a 5’  3’ direction • DNA polymerase can add only at the 3’ end • Nucleotides are polymerized and 2 phosphates are removed in the process

  30. Nucleotide Synthesis • Raw nucleotides are nucleoside triphosphates • N base, deoxyribose, & a triphosphate tail • Nucleotide added, last 2 phosphate grps hydrolyzed, forming pyrophosphate • Exergonic rxn drives polymerization of the nucleotide to the new strand

  31. DNA Pol • DNA polymerases can only add nucleotides to the free 3’ end of a growing DNA strand • A new DNA strand can only elongate in the 5’  3’ direction

  32. Problems? • One parental strand is oriented 3’  5’ into the fork, while the other is oriented 5’  3’ into the fork • At fork, only one parental strand (3’  5’ into the fork), leading strand, can be used by polymerases as a template for a continuous complementary strand

  33. Continuous & Discontinuous

  34. Continuous & Discontinuous • Replication is continuous on one strand and discontinuous on the other • Replication begins at replication forks

  35. Okazaki fragments • Synthesis of the leading strand is continuous • The lagging strand (discontinuous) is synthesized in pieces called Okazaki fragments

  36. Okazaki fragments • 100 - 1000 nucleotides in length • Initiated by a separate RNA primer • Okazaki fragments are joined together by DNA ligase

  37. RNA Primer • DNA pol cannot initiatesynthesis because it can only add nucleotides to end of an existing chain • Requires an RNA primer • Primase, an RNA pol, links ribonucleotides complementary to the DNA template into the primer • RNA pol can start an RNA chain from a single template strand

More Related