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University of Houston BCHS 3304: General Biochemistry I - Fall 2008 Section 12697

This University of Houston course covers condensation reactions, chemical evolution, replication through complementarity, Gibb's free energy, the van't Hoff relationship, nonpolar/polar interactions, Henderson-Hasselbalch equation, Fischer convention, and cleavage by CNBr and trypsin.

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University of Houston BCHS 3304: General Biochemistry I - Fall 2008 Section 12697

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  1. University of Houston BCHS 3304: General Biochemistry I - Fall 2008 Section 12697 Tuesday/Thursday 11:30 AM – 1:00 PM 102 SW Exam 1 Overview

  2. Condensation reactions • Chemical Evolution, simple molecules condense to form more complex forms (polymers) • Reaction of a carboxylic acid with an amine

  3. Replication through complementarity • Specific pairing of functional groups gives rise to complementarity • More complex molecules increases chemical versatility • Complementarity makes it possible for macromolecules to replicate • Over time natural selection favored molecules that made accurate copies of themselves

  4. Gibb’s Free Energy • Is a state function (a property of a system that depends only on the current state of the system and not its history) • Gibb’s Free Energy is determined at constant T and P: • The Gibbs free energy (G) of a system is defined by an enthalpy term (H) (change of the total energy with the system), and the entropy term (S) (change in the disorder) at temperature (T) G = H - TS G = H - TS

  5. The van’t Hoff Relationship • Methodology of finding ΔH and ΔS from experimental data. Van’t Hoff plot = Slope lnKeq = Intercept

  6. Structure & Properties of Water Bent geometry, O-H bond length of 0.958Å Can form Hydrogen bonds

  7. Nonpolar/Polar Interactions and Structured Water A cage of water molecules (calatherate) surrounding the non-polar molecule. This cage has more structure than the surrounding bulk media. DG = DH -TDS To minimize the structure of water the hydrophobic molecules cluster together minimizing the surface area.

  8. Henderson - Hasselbalch equation From Rearrange Take (-)Log of each

  9. Titration curve for phosphate

  10. Key to structure (1) G A V L I M P F W

  11. S T N Q Y C (2)

  12. K R H D E (3)

  13. The Fischer Convention Absolute configuration about an asymmetric carbon related to glyceraldehyde (+) = D-Glyceraldehyde (-) = L-Glyceraldehyde

  14. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 H3N+-_-_-_-_-_-_-_-_-_-_-_-_-_-_-_-COO- A-T F - M - A - T A- K - F - M Q - M -A - K D - I - K - Q - M G - M - D - I - K Y - R - G - M Y - R Cyanogen Bromide (CNBr) Cleaves after Met i.e M - X D - I - K - Q - M A - T A - K - F - M Y - R - G - M Trypsin cleaves after K or R (positively charged amino acids) Q - M - A - K G - M - D - I - K F - M - A - T Y - R

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