1 / 10

Micropipette Lab

Micropipette Lab. Comparing Precision and Accuracy!. Cornell Notes & INB’s. Q column: Students write questions from notes Everything in this column should be a question. S - Set up the paper T - Take Notes A - After class process the notes 3 summary statements 1 questions

valmai
Download Presentation

Micropipette Lab

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Micropipette Lab Comparing Precision and Accuracy!

  2. Cornell Notes & INB’s Q column: Students write questions from notes Everything in this column should be a question • S - Set up the paper • T - Take Notes • A - After class process the notes • 3 summary statements • 1 questions • R - Reflect / review

  3. Precision • The precision of a measurement describes the “units” used to measure something • The smaller the unit, the more precise!

  4. Example of Precision Ruler #1 can measure in centimeters “The pencil is a little over 9 cm” Ruler #2 can measure in millimeters “The pencil is a little over 95 millimeters Ruler #2 Which ruler is more precise???

  5. Which tool can measure volume more precisely? How about compared to this tool? 20µL = .020 ml 10 ml, 50 ml, or 100 ml graduated cylinder?

  6. Accuracy • The difference between your measurement and the “accepted” value • The bigger the difference, the less accurate you were!

  7. Examples of Accuracy Bob measured this container of milk And determined that it is 5.5 gallons Bob wasn’t very accurate! Maria measured this container of milk And determined that it is 1.1 gallon Maria was very accurate! Mai measured this container of milk And determined that it is 3.7854118 Liters = 3.7854118 liters = 1 gallon These are accepted values Mai was accurate and precise!

  8. Rules when using a micropipette • Gently remove the disposable tip by depressing the button (not the top) • Never use a micropipette without a tip • Never lay a loaded pipette down or tilt it sideways because this could allow liquid to run back into the instrument • Never let the button snap back into place, always allow it to rise slowly back into position

  9. Let’s practice! • Gently add a tip by pushing it onto the end of the pipette • Slowly turn the top knob to set the desired measurement • 200 is 200, 20 is 020 (large volume pipette) • 20 is 200, 2 is 020 (small volume pipette) • Slowly depress top knob down until you reach the “first stop” • Slowly put tip of pipette into water and slowly release knob • Depress top knob until you reach to “second stop” to release liquid

  10. Precision

More Related