1 / 10

Triacylglycerol Synthesis

O. O. O. R-C-O. O-C-R. O-C-R. Triacylglycerol Synthesis. Fatty acyl-CoA DHAP reduction to glycerol-PO 4 or Glycerol kinase to glycerol-PO 4 Two esterifications Diacylglycerol-PO 4 intermediate Triacylglycerol. glycolysis. NADH + NAD +. ADP ATP. CH 2 OH. C=O. CH 2 OP.

unity
Download Presentation

Triacylglycerol Synthesis

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. O O O R-C-O O-C-R O-C-R Triacylglycerol Synthesis • Fatty acyl-CoA • DHAP reduction to glycerol-PO4 or • Glycerol kinase to glycerol-PO4 • Two esterifications • Diacylglycerol-PO4 intermediate • Triacylglycerol

  2. glycolysis NADH + NAD+ ADP ATP CH2OH C=O CH2OP CH2OH CH2OH O O Not in adipose tissue O O O 2 HO-C-H HO-C-H R-C~CoA R-C~CoA CH2O-C-R CH2O-C-R CH2O-C-R O O O CH2OH CH2OP R-C-O-C-H R-C-O-C-H R-C-O-C-H CH2OH CH2OP CH2O-C-R H2O PO4 O Triacylglycerol Biosynthesis glycerol kinase glycerol-PO4 dehydrogenase DHAP Glycerol-PO4 Phosphatidic acid Phospholipid biosynthesis 1,2 Diacylglycerol (DAG)

  3. Question • Can a triacylglycerol (triglyceride) storage fat be synthesized entirely from glucose, i.e., every carbon in the fat comes from a sugar? • Answer: YES

  4. Phosphatidic Acid O - or + - or + O O O Ester linkage O O CH2O-C-R CH2O-C-R O O R-C-O-C-H R-C-O-C-H CH2OPO-CH2CH2-N(CH3)3 CH2OP O O Phospholipid Biosynthesis (smooth ER) Polar component = choline, serine, ethanolamine, etc Glycerophospholipids + Phosphatidylcholine or lecithin

  5. PPP- Ribose CTP Strategy of Glycerophospholipid Biosynthesis • Activate diacylglycerol • Activate appending moiety (salvage)

  6. O CH2O-C-R O R-C-O-C-H CH2OP~P-ribose-Cytosine (CDP-diacylglycerol) Bacteria (Prokaryotes) Activate Phosphatidic acid CDP-diacylglycerol + PPi Phosphatidic acid + CTP

  7. Serine Glycerol-PO4 CMP CMP Phosphatidyl- glycerol CO2 glycerol Wassermann antigen for diagnosing syphilis Most prominent membrane lipid in bacteria Bacteria (Prokaryotes) CDP-diacylglycerol Phosphatidylserine Phosphatidylglycerol-3-PO4 Phosphatidylethanolamine Cardiolipin

  8. 1 2 3 O O CH2O-C-R CH2O-C-R O O R-C-O-C-H R-C-O-C-H CH2OP CH2OH Pi Eukaryotes ATP Glycerol Glycerol-3-PO4 DHAP FA-CoA 1-Acyl-DHAP Phosphatidic acid NADPH DAG 1-Acyl-glycerol-3-PO4 ATP CTP CDP-diacylglycerol ethanolamine (CDP-ethanolamine) choline (CDP-choline) Serine (phosphatidylethanolamine) Glycerol (CDP-diacylglycerol) Inositol (CDP-diacylglycerol) 1,2 DAG Cardiolipin (phosphatidylglycerol)

  9. N H 2 N O O O N + H O P N C H C H O P O C H 2 2 2 3 O O O H O ethanolamine O H C y t i d i n e d i p h o s p h a t e ( C D P )

  10. ATP ADP O HO-P- O-CH2-CH2-NH3+ O- CTP PPi O O Cytidine- -P- O-P- O-CH2-CH2-NH3+ O- O- DAG CMP Serine Ethanolamine SALVAGE Ethanolamine or choline HO-CH2-CH2-NH3+ Ethanolamine kinase or choline kinase Phosphoethanolamine CDP-ethanolamine Phosphatidylethanolamine Phosphatidylserine

More Related