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Surface modification using Nanoparticle to inhibit cellular proliferation

Surface modification using Nanoparticle to inhibit cellular proliferation. B.G.Cousins , P.J.Doherty , , M.J.Garvey , R.L.Williams University of Liverpool U.K. OPAL. 1 micron. Diatoms Opal.

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Surface modification using Nanoparticle to inhibit cellular proliferation

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  1. Surface modification using Nanoparticle to inhibit cellular proliferation B.G.Cousins, P.J.Doherty,, M.J.Garvey, R.L.Williams University of Liverpool U.K.

  2. OPAL 1 micron

  3. Diatoms Opal • The subunits of colloidal silica are non-porous, spherical particles of ~10nm • Silica is present in all connective tissues (collagen, arterial cell walls) along with nails, skin and hair • Exists in nature in plants, diatoms and opal

  4. Colloidal Silica aggregates in aqueous dispersion to form 3-dimensional irreversible gels Silica Sol Silica Gel Irreversible Network Dissolution/ Deposition

  5. Interface “Interfacial Gelation” of partially hydrophobed silica at an air or hydrophobic interface. Low levels of adsorbed cationic surfactant render colloidal silica surface active.

  6. Two-dimensional aggregates silica 0.1 micron 2 micron

  7. Air Water Silica Sol Monolayer Silica Sol Interfacial Gelation of partially hydrophobed silica at the air/water interface. LANGMUIR-BLODGETT Type Deposition

  8. Electron micrograph of interfacial film of 14 nm silica particles

  9. 50 micron Interfacial rigidity of silica coating, observed at an oil/water interface Non-spherical oil droplets formed in cationic coated silica dispersion Rigid interface

  10. MRC Discipline Hopper Award Deposition of Nanoparticles for Control of Cellular Response to Surfaces • Cells respond to nanoscale structures and adapt to topography • Deposition of nanoparticles offers a simple route to topographical modification. Potential applications in wound dressing, hygienic surfaces and implants.

  11. Study Aim • The aim of the study was the manipulation of the surface topography to influence cellular response • Approach uses the deposition of commercially available, nanoscale, silica particles to modify the topography • Cationic surfactant coated silica limited to hydrophobic surfaces and necessitated an alternative deposition process.

  12. ZetagTMPrimary Adhesive Layer N+R3 N+R3 N+R3 N+R3 N+R3 N+R3 N+R3 N+R3 N+R3 N+R3 N+R3 O- O- O- O- O- OH OH OH OH OH OH O- • Ionic adsorption of Zetag on to a glass surface is achieved via (O- N+R3) ion-pairs

  13. SiO2 SiO2 SiO2 SiO2 SiO2 SiO2 SiO2 SiO2 + + + + + + + + Deposition of Silica • Negative silica particles adsorb on to the cationic polymer

  14. Results • 21 nm Silica particles attached to glass substrate • Image is representative of 7, 14, 21 and 80 nm silica particles

  15. Core technology Intraocular lenses Wound dressings Surface Modification Using Nanotechnology Bone fracture repair devices Potential Applications of Core Technology

  16. Plain glass Plain glass Nanoparticles Nanoparticles Cell Response to Nanotopography • Fibroblast cells on coated and uncoated glass • Silica particles inhibit adhesion/spreading of cells • Silica particles prevent migration of cells • This effect is not altered by changing protein concentration • Effect seen with other cell types (primary & established)

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