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Stem Cell Science. A brief update 9/10/08 Gerry Maxwell. Jaenisch and Young 2008 Cell 132:567-582. Jaenisch and Young 2008 Cell 132:567-582. I. Park and G.Q. Daley 2007 Nature Cell biology 9: 871-873. Jaenisch and Young 2008 Cell 132:567-582. Kinetics of reprogramming.

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stem cell science

Stem Cell Science

A brief update

9/10/08

Gerry Maxwell

kinetics of reprogramming
Kinetics of reprogramming
  • Reprogramming using virally encoded transcription factors in vitro takes many days and not all markers of pluripotency are activated together
  • In addition, it appears that stochastic events are involved in reprogramming because clonally derived cells do not reprogram in a synchronous fashion
creation of patient specific cell lines
Creation of Patient Specific Cell Lines
  • Originally published in Science Express on 31 July 2008
  • Science 29 August 2008:
  • Vol. 321. no. 5893, pp. 1218 - 1221
  • DOI: 10.1126/science.1158799
  • Induced Pluripotent Stem Cells Generated from Patients with ALS Can Be Differentiated into Motor Neurons
  • John T. Dimos,1* Kit T. Rodolfa,1,2* Kathy K. Niakan,1 Laurin M. Weisenthal,1 Hiroshi Mitsumoto,3,4 Wendy Chung,4,5 Gist F. Croft,4,6 Genevieve Saphier,1 Rudy Leibel,5 Robin Goland,7 Hynek Wichterle,4,6 Christopher. Henderson,4,6 Kevin E Eggan1
slide9

In vivo reprogramming of adult pancreatic exocrine cells tocellsZhou,Brown, Kanareck, Rajagopal, and Melton

2008 Nature 07134

slide10

A combination of three transcription factors induces

insulin+ cells in adult mouse pancreas in vivo.

Q Zhouet al.Nature000, 1-6 (2008) doi:10.1038/nature07314

slide11

Induced new b-cells remodel vasculature and

ameliorate hyperglycaemia.

Q Zhouet al.Nature000, 1-6 (2008) doi:10.1038/nature07314

characteristics of in vivo reprogramming
Characteristics of in vivo reprogramming
  • Like in vitro the inducing factors are only required transiently
  • Does not appear to require cell division
  • Occurs as early as 3 days-faster than in vitro (7-30 days in vitro)
  • Efficiency is up to 20%- much higher than in vitro
  • Does not appear to involve dedifferentiation as does in vitro reprogramming