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計畫編號: 100-B1-1 主持人:李桂楨、李振綱 計畫領域別:生命科學 - 工程 PowerPoint Presentation
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計畫編號: 100-B1-1 主持人:李桂楨、李振綱 計畫領域別:生命科學 - 工程

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計畫編號: 100-B1-1 主持人:李桂楨、李振綱 計畫領域別:生命科學 - 工程 - PowerPoint PPT Presentation


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跨領域 『 蛋白質工程 』 專題學習 利用螢光顯微影像及表面電漿共振分析 小分子化合物抑制 amyloid- b 聚集的情形. 計畫編號: 100-B1-1 主持人:李桂楨、李振綱 計畫領域別:生命科學 - 工程.

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100 b1 1

跨領域『蛋白質工程』專題學習

利用螢光顯微影像及表面電漿共振分析

小分子化合物抑制amyloid-b聚集的情形

計畫編號:100-B1-1

主持人:李桂楨、李振綱

計畫領域別:生命科學-工程

slide3

Ab42-GFP基因選殖

(HindIII-BamHI片段)

(BamHI- EcoRI片段)

(EcoRI-NotI片段)

slide4

His6-Trx-Ab42基因選殖

Ab42

Trx (thioredoxin) Tag

slide5

DMSO

小分子抑制物

錯誤摺疊、無螢光 正確摺疊、有螢光

Congo red Curcumin

真核細胞中Ab42-GFP與小分子抑制物結合之螢光顯微影像觀察

(Congo red,

Curcumin)

slide6
Ni++ chelating agent (IDA or NTA) grafted onto sensor surface

Ni++ chelated on the surface

His6-Trx-Ab42 immobilized via metal affinity interaction between His6 and immobilized Ni++

Response monitored if inhibitor captured by immobilized His6-Trx-Ab42

His6-Trx-Ab42與小分子抑制物的表面電漿共振分析