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AFCG Biosafety Guidelines for Analysis and Sorting of Unfixed Cells.

AFCG Biosafety Guidelines for Analysis and Sorting of Unfixed Cells. AFCG website. Scope This document RECOMMENDS procedures to be undertaken during the analysis and sorting of potentially biohazardous material on Flow Cytometers. AFCG Guidelines 2006 - Introduction Page 2 of guidelines

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AFCG Biosafety Guidelines for Analysis and Sorting of Unfixed Cells.

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  1. AFCG Biosafety Guidelines for Analysis and Sorting of Unfixed Cells. AFCG website ScopeThis document RECOMMENDSprocedures to be undertaken during the analysis and sorting of potentially biohazardous material on Flow Cytometers.

  2. AFCG Guidelines 2006 - Introduction Page 2 of guidelines AFCG website FOREWORD These recommendations are presented with a view to being a minimum standard and should not be seen to restrict the ability of any individual. This document will be reviewed regularly to ensure that these recommendations embrace current accepted laboratory practices. These guidelines are written in broad terms as a gesture to indicate and not to dictate. HOWEVER

  3. EMPLOYER RESPONSIBILITIES SO FAR AS IS “REASONABLY PRACTICABLE”: Provide and maintain a safe • working environment • plant or systems of work • workplace in a condition that is safe and without risk • system for the use, handling, storage or transport of plant or substances

  4. Biohazardous material · All unfixed material of human origin. · All unfixed material of primate origin. · All human peripheral blood leukocytes, bone marrow, splenocytes, thymocytes, sperm cells, · Cells from primary and immortalized cultures from humans, primates, and transgenic animals. · All genetically modified organisms (GMO) · Micro-organisms. · Special care to be taken for brain tissue (prions) and lung lavage (tuberculosis). WHY ? TO PROTECT THE OPERATOR , THE USERS AND THE ENVIROMENT.

  5. GENERAL CONSIDERATIONS • Policies

  6. GENERAL CONSIDERATIONS 2) BEFORE A NEW USER STARTS: Flow Cytometry tutorials are mandatory, they cover theory practice and safety.

  7. GENERAL CONSIDERATIONS 3) NOTIFICATION

  8. Implementation Handling of biohazardous material >> IN TRANSIT AND PREPARATION STANDARD PC2page 2 AFCG Guidelines

  9. Implementation REFERENCES: page 14 of guidelines · www.ogtr.gov.au/ · Biosafety Guidelines for Sorting of Unfixed Cells, Cytometry, 28:99-117 (1997). · Cytometry A. 2007 Jun; 71(6):414-37. International Society for Analytical Cytology biosafety standard for sorting of unfixed cells. Schmid I, Lambert C, Ambrozak D, Marti GE, Moss DM, Perfetto SP;International Society of Analytical Cytology. · Biosafety Considerations for Flow Cytometric Analysis of HIV infected Samples, Cytometry Comm., 38:195-200 (1999). · Novel Rapid Method for Visualization of Extent and Location of Aerosol Contamination During High-Speed Sorting of Potentially Biohazardous Samples, Cytometry, 42:217-22 (2001).· Measuring Containment of Viable et Infectious Cell Sorting in High-Velocity Cell Sorters. Perfetto et al., Cytometry Part A 52A:122-130(2003) · Viable Infectious Cell Sorting in a BSL-3 Facility, Flow Cytometry Protocols, vol. 263, Humana Press, 2004 · Biohazard Sorting, Methods in Cell Biology, Cytometry, 4th ed., vol.75,2004Oberyszyn A.S. & Robertson F.M. .Novel rapid method for visualization of extent and location of aerosol Contamination during high speed sorting of potentially biohazardous samples. Cytometry 2001; 43: 217-222 · Current Protocols in Cytometry Jan 2007: UNIT 3.6 Standard Safety Practices for Sorting of Unfixed Cells. Ingrid Schmid1, Claude Lambert2, David Ambrozak3, and Stephen P. Perfetto3 .Current Protocols in Cytometry Feb 2002 UNIT 3.5 Method for Visualizing Aerosol Contamination in Flow Sorters. Andrew S. Oberyszyn1 .WHO Biosafety Manual.http://www.who.int/csr/resources/publications/biosafety/who

  10. Implementation / Testing

  11. Implementation / Testing • “IN HOUSE PROCEDURES”

  12. SURVEILLANCE • “IN HOUSE PROCEDURES”> work practices • Passaged cells , how many times • New work , ask what type • Unsure , ask IBC Ask the question

  13. MANAGEMENT OHS requirements IBC Internal Biosafety Committee AFCG guidelines Line manager

  14. Decontamination Procedures

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