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June 12th-14th, 2013. Collaborative E - L earning application for electro p oration - based biomedical applications. Selma Corovic. University of Ljubljana, Faculty of Electrical Engineering Slovenia. Background and motivation.

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Presentation Transcript
slide1

June 12th-14th, 2013

Collaborative E-Learning application for electroporation-based biomedical applications

Selma Corovic

University of Ljubljana,

Faculty of Electrical Engineering

Slovenia

slide2

Background and motivation

  • Electroporation of cells and tissues – human, animal or plant cells for different biomedical and biotechnological applications
  • Electroporation based biomedical applications require multidisciplinary expertise: oncologists, physicists, engineers…

clinical electrochemotherapy - cancer treatment

Collaborative E-Learning application

Oncology

Biomedical engineering

University of Ljubljana

slide3

E-Learning application

Collecting

the knowledge

E-learning application on

cells and tissues

Designing

the content

Research

+

Clinical trials

E-Learning enviroment

(EBTT)

Improving the content

based on results

Evaluation (pedagogical efficiency and usability)

slide4

E-Learning enviroment

Electroporation based technologies and treatments

International SCIENTIFIC WORKSHOP and POSTGRADUATE COURSE

November 18-24, 2012, Ljubljana, Slovenia

Lectures

Practical work

Lectures were recorded

electroporation

cell membrane

molecules

Electroporation

Important parameter for effective electroporation

local electric field distribution E [V/cm]!

slide6

Local electric field distributionE >Erev

Electroporated

Cell membrane

Cell

Tissue

slide7

Applications of Electroporation

Electroporation is used in:

- clinical electrochemotherapy, non-viral gene electrotransfer for gene therapy and DNA vaccination, transdermal drug delivery…

- liquid food sterilization, water treatment, electrofusion of cells…

biomedical

UTI = −1.5 rE cos (φ)

  • The electroporation of cell membrane first occurs within the cell area facing the electrodes
  • Different electroporation regimes:
  • reversible Erev < E < Eirrev and irreversible E >Eirrev
electrochemotherapy chemotherapy electroporation
Electrochemotherapy = = chemotherapy + electroporation

Electric pulse generator

Chemotherapeutic surrounds the cells

Increased membrane permeability allows access to the cytosol

Pores reseal

Electrodes

time

Tumour

Electric pulse application

Injecting chemotherapeutic

slide9

Protocols and local electric field distribution in tumors

Protocols used in clinical electrochemotherapy today include:

8 pulses of 100 µs duration, f=1 Hz or f=5 KHz, U/d =1300 V/cm

drug

injection

+

Result

the entire volume of the tumor

needs to be subjected to the E ≥ Erev

Local electric field distribution:

U/d

slide10

the entire volume of the tumor

needs to be subjected to the E ≥ Erev

Importance of an adequate local electric field for

successful tissue electroporation (example: unsuccessful tumor treatment)

slide11

Clinical use of electrochemotherapy

Reimbursed in:

Germany, UK, Italy, Portugal, Denmark, Greece,Slovenia.

Poland – in progress for reimbursement. ECT approved by Minister of Health.

slide13

E-Learning application

Collecting

the knowledge

E-learning application on

cells and tissues

Designing

the content

Research

+

Clinical trials

E-Learning enviroment

(EBTT)

Improving the content

based on results

Evaluation (pedagogical efficiency and usability)

laboratory of bio cybernetics
Laboratory of biocybernetics

http://lbk.fe.uni-lj.si

Lea Retelj, Ajda Bicek, Matevz Leskovsek, Jasna Krmelj, Tadej Kotnik, Dusa Hodzic, Marko Usaj, Gorazd Pucihar, Alenka Macek Lebar, Samo Mahnic, Barbara Mali, Tomaz Jarm, Jaka Cemazar, Selma Corovic, Denis Pavliha, Bor Kos, Masa Kanduser, Natasa Pavselj, Janez Zigon, Ivan Pavlovic, Stanislav Rebersek, David Slavic, Melvin Essone, Jure Jelenc, Matej Rebersek, Tina Batista Napotnik, Mitja Gliha, Sasa Haberl, Peter Kramar, Igor Marjanovic, Barbara Zorec, Matej Kranjc, Damijan Miklavcic.

(Missing from the photo: Karel Flisar, Marija Marcan, Andraz Polak)