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RNA and protein synthesis

RNA and protein synthesis. RNA. Single strand of nucleotides Sugar is ribose Uracil instead of thymine. DNA provides workers with the instructions for making the proteins and the workers build the proteins Other workers bring parts, the amino acids, over to the assembly line

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RNA and protein synthesis

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  1. RNA and protein synthesis

  2. RNA • Single strand of nucleotides • Sugar is ribose • Uracil instead of thymine

  3. DNA provides workers with the instructions for making the proteins and the workers build the proteins • Other workers bring parts, the amino acids, over to the assembly line • The workers for protein synthesis are RNA molecules, which take the instructions from DNA and assemble the protein amino acid by amino acid

  4. mRNA • Messenger RNA • Brings information from the DNA in the nucleus to the cell’s factory floor, the cytoplasm

  5. rRNA • Ribosomal RNA • Clamp onto the mRNA and use its information to assemble the amino acids in the correct order

  6. tRNA • Transfer RNA • Is the supplier • Transports amino acids to the ribosome to be assembled into a protein

  7. Transcription • mRNA carries information from the DNA to the cell’s ribosomes for manufacturing proteins • In nucleus enzymes make an RNA copy or a portion of a DNA strand during transcription

  8. Enzymes unzip the DNA molecule • Free RNA nucleotides pair with complementary DNA nucleotides on one of the DNA strands • When base pairing is completed, mRNA molecule breaks away as DNA strands rejoin. mRNA leaves the nucleus and enters the cytoplasm

  9. The nucleotide sequence transcribed from DNA to a strand of mRNA acts as a genetic message, the complete information for the building of a protein. • Message is written in nitrogen bases.

  10. Codon • A group of 3 nucleotides on mRNA that codes for one amino acid

  11. The order of nitrogen bases in the mRNA will determine the type and order of amino acids in a protein. • 64 combinations are possible therefore 64 different mRNA codons • Some codons do not code for amino acids; they provide instructions for assembling the protein. • UAA = Stop codon • AUG = start codon

  12. Genetic code • All organisms use the same genetic code for amino acids and assembling proteins • Genetic code is universal

  13. Translation • The process of converting the information in a sequence of nitrogen bases in mRNA into a sequence of amino acids • Takes place at ribosomes in the cytoplasm

  14. Anticodon • Correct translation of the mRNA message depends upon the joining of each mRNA codon with the correct tRNA molecule • On tRNA molecule there is a sequence of 3 nucleotides that are the complement of the nucleotides in the codon, which are called an anticodon.

  15. Translation 1. tRNAmolecule brings the first amino acid to the mRNA strand that is attached to the ribosome. 2. Anticodon forms a temporary bond with the codon of the mRNA strand

  16. 3. The ribosomes slides down the mRNA chain to the next codon and a new tRNA molecule brings another amino acid 4. The amino acids bond, the first tRNA releases its amino acid and detaches from the mRNA 5. The tRNA molecule is now free to pick up and deliver another molecule of its specific amino acid to a ribosome

  17. 6. The ribosome slides down to the next codon; a new tRNA molecule arrives and its amino acid bonds to the previous one 7. A chain of amino acids begins to form 8. When a STOP codon is reached, translation ends and the amino acid strand is released from the ribosome

  18. Protein Synthesis Summary • Creation of proteins • 2 part process – transcription and translation • mRNA brings message from DNA in nucleus to the cytoplasm • Ribosome binds to mRNA and tRNA brings in amino acids which bond together to form a protein. • Codon and anticodon must be complementary

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