Download
slide1 n.
Skip this Video
Loading SlideShow in 5 Seconds..
Ang II (+) PowerPoint Presentation

Ang II (+)

127 Views Download Presentation
Download Presentation

Ang II (+)

- - - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript

  1. Ang II (–) Ang II (+) SA-b-gal DAPI Supplemental Figure 1

  2. A 1 AngII (+) AngII (–) * * 0.8 * 0.6 Aldosterone concentration (nM) * 0.4 * * 0.2 * 0 1 2 3 4 5 6 7 (days) Supplemental Figure 2A

  3. 80 * 60 * SA-b-gal-positive cells (% of total cells) * 40 20 0 0 0.2 0.4 0.8 1 Aldo (nM) 16 B C p21 * 12 * Fold change 8 * * 4 0 4 p53 * * p21 Fold change 2 p53 0 p16 p16 * 6 * * p27 4 Fold change a-SM actin * 2 0 0.2 0.4 0.8 1 0 6 p27 * Aldo (nM) * 4 * Fold change * 2 0 0 0.2 0.4 0.8 1 Supplemental Figure 2B,2C Aldo (nM)

  4. Ki-ras2A a-SM actin 5 * * 4 * Ki-ras2A (fold change) 3 * 2 1 0 AngII+Aldo 0 1 3 5 7 (days) Supplemental Figure 3

  5. Name Sequence S 5’- CGAAACAGGCUCAGGAGUUTT-3’ Ki-ras2A-1 AS 5’- AACUCCUGAGCCUGUUUCGTT-3’ S 5’-CCGAGAAAUUCGAAAACAUTT -3’ Ki-ras2A-2 AS 5’- AUGUUUUCGAAUUUCUCGGTT-3’ S 5’-AAGAAGAAGUCAAGGACAATT -3’ Ki-ras2A-3 AS 5’- UUGUCCUUGACUUCUUCUUTT-3’ Supplemental Table 1: Sequences for cocktail of siRNA targeting Ki-ras2A Supplemental Table 1

  6. Name Sequence S 5’- GCGCCGUACGAGGUAUAAATT-3’ Scrambled control-1 AS 5’- UUUAUACCUCGUACGGCGCTT -3’ S 5’-AUAGAACGACAACGCUAUATT -3’ Scrambled control-2 AS 5’- UAUAGCGUUGUCGUUCUAUTT-3’ S 5’-CGAUUAAGAAACGAAUAGGTT -3’ Scrambled control-3 AS 5’- CCUAUUCGUUUCUUAAUCGTT-3’ Supplemental Table 2: Sequences for cocktail of scrambled version of Ki-ras2A- siRNA as control Supplemental Table 2