Stochasticity in Signaling Pathways and Gene Regulation: The NF κ B Example and the Principle of Stochastic Robustness. Marek Kimmel Rice University, Houston, TX, USA. Rice University Pawel Paszek Roberto Bertolusso UTMB – Galveston Allan Brasier Bing Tian Politechnika Slaska
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Rice University, Houston, TX, USA
Negative autoregulation of the NF-kB pathway
Time-continuous Markov chain with state space
and transition intensities
only gene on/off discrete stochastic
Any single cell trajectory differs from the “averaged” trajectory
TF turnover ~10 sec
Transcription of the gene occurs in bursts, which are asynchronous in different cells.
Parameters estimated by fitting the distribution of the level of nuclear message, apparently contradict photobleaching experiments.
Amount of protein:
The system has one or two stable equilibrium points depending on the parameters.