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5’ 3’ - PowerPoint PPT Presentation


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H19 DMR. 5’ 3’ GTAGATTTGGTTATAGTTAAATGGATA----ATTTTATAGTTATAAAAGTAGAGGTTGG CATCTAAACCAATATCAATTTACCTAT----TAAAATATCAATATTTTCATCTCCAACC 3’ 5’. PCR.

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Presentation Transcript
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H19 DMR

5’ 3’

GTAGATTTGGTTATAGTTAAATGGATA----ATTTTATAGTTATAAAAGTAGAGGTTGG

CATCTAAACCAATATCAATTTACCTAT----TAAAATATCAATATTTTCATCTCCAACC

3’ 5’

PCR

5’ 3’

GTAGATTTGGTTATAGTTAAATGGATA----ATTTTATAGTTATAAAAGTAGAGGTTGG

Converted top strand

Bisulfite

5’ 3’

GCAGATTTGGCTATAGCTAAATGGACA----ACCCCATAGCCATAAAAGCAGAGGCTGG

CGTCTAAACCGATATCGATTTACCTGT----TGGGGTATCGGTATTTTCGTCTCCGACC

3’ 5’

(Beware of CpG dinucleotides in primer sequence)

Bisulfite

TGTTTAAATTGATATTGATTTATTTGT----TGGGGTATTGGTATTTTTGTTTTTGATT

3’ 5’

Converted bottom strand

PCR

5’ 3’

ACAAATTTAACTATAACTAAATAAACA----ACCCCATAACCATAAAAACAAAAACTAA

TGTTTAAATTGATATTGATTTATTTGT----TGGGGTATTGGTATTTTTGTTTTTGATT

3’ 5’