effect of calorie restriction on doxorubicin induced cardiac dysfunction n.
Download
Skip this Video
Loading SlideShow in 5 Seconds..
Effect of Calorie Restriction on Doxorubicin Induced Cardiac Dysfunction PowerPoint Presentation
Download Presentation
Effect of Calorie Restriction on Doxorubicin Induced Cardiac Dysfunction

Loading in 2 Seconds...

play fullscreen
1 / 14

Effect of Calorie Restriction on Doxorubicin Induced Cardiac Dysfunction - PowerPoint PPT Presentation


  • 137 Views
  • Uploaded on

Effect of Calorie Restriction on Doxorubicin Induced Cardiac Dysfunction. Celina Lucero Genysie Van Duren. Background: Doxorubicin (DOX). Potent Chemotherapy Effective against: Solid tumors Leukemia breast cancer Destroys cells by: Apoptosis DNA damage. L eukemia. DNA damage.

loader
I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
capcha
Download Presentation

PowerPoint Slideshow about 'Effect of Calorie Restriction on Doxorubicin Induced Cardiac Dysfunction' - tod


An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript
effect of calorie restriction on doxorubicin induced cardiac dysfunction

Effect of Calorie Restriction on Doxorubicin Induced Cardiac Dysfunction

Celina Lucero

Genysie Van Duren

background doxorubicin dox
Background: Doxorubicin (DOX)
  • Potent Chemotherapy
  • Effective against:
    • Solid tumors
    • Leukemia
    • breast cancer
  • Destroys cells by:
    • Apoptosis
    • DNA damage

Leukemia

DNA damage

background dox
Background: DOX
  • Problem:
    • Attacks all metabolically active cells
    • Damages cardiac tissue
    • Creates reactive oxygen species and oxidative stress
  • Side Effects:
    • Cardiomyopathy
    • Severe acute illnesses
  • “Red Death”

Cardiac tissue

background calorie restriction cr
Background: Calorie Restriction (CR)
  • Form of dieting
  • Proven to:
    • Extend lifespan
    • Preserves cardiac function
    • Lower body weight
  • Differing Effects:
    • Acute= negative results
    • Chronic= positive results
introduction
Introduction
  • Used rats:
    • Split into 4 groups-
      • Ad Lib (AL)+Saline (SAL), AL+DOX, CR+SAL, and CR+DOX
  • Done over 4 weeks
  • Echocardiographs monitor heart:
    • M-mode= left ventricle
    • Doppler= mitral and aortic valves

Ekg: echocardiogram

hypothesis purpose
Hypothesis/Purpose
  • Known facts:
    • DOX= effective…but dangerous
    • CR= prolong heart functions
  • Combination?
    • Both together= beneficial?
    • Solve adverse effects of DOX
    • Safer use with patients
methods
Methods
  • Used Sprague-Dawley(Rattusnorvegicus) rats.
    • Reliable and healthy subjects
  • Feeding:
    • Normal first 2 weeks
    • CR or AL treatments follow initial 2 weeks.
      • CR= 60% of AL intake
methods1
Methods
  • Timeline Instructions
  • At 10 wks animals (males and females) assigned to feeding and activity regimens.
  • After a 2 week acclamation the DOX treatment will start
  • On weeks 2-9 the animals will receive either saline or DOX at 2.5 mg/kg for a total dose of 20 mg/kg
results
Results
  • Body Mass:
    • General decrease. CR= more
    • No significant differences
  • M-mode:
    • Variables generally the same
    • Corrected velocity of circumferential shortening (Vcfa) was only significant difference.
      • Between CR+DOX and AL+DOX
  • Doppler:
    • Variables generally the same
    • No significant differences
results1
Results

Table 1.Body Weight Averages

Average body weights (g) over the course of the experiment. Number in parenthesis represent the standard deviation (StDev).

results2
Results

Percentage

*

mm/sec

Fractional shortening at week 4. Percentage of wall change during diastole to systole.

Vcfa at week 4. Speed at which the percentage change from FS happens adjusted to heart size.

*The black portion of the graph represents the standard error.

Diastole= relaxation

Systole= contraction

discussion
Discussion
  • Body Mass:
    • CR= more weight loss
    • DOX did not effect weight
      • DOX normally lowers weight
  • M-mode:
    • Little thinning in LV walls
    • Previous study= profound thinning in LV walls
  • Doppler:
    • No significant differences
  • Fatalities:
    • Two rats in DOX group

Top: M-mode

Bottom: Doppler

recommendations
Recommendations
  • Time Constraints:
    • Chronic CR proven to be better
      • Rats did not have enough time.
    • Only 7.5 mg/kg of DOX injected cumulatively
      • Previous studies= larger amount DOX
      • Again longer period of time
        • Rats not exposed to DOX long enough
  • Subjects:
    • Small sample size, decreased power
acknowledgments
Acknowledgments
  • Thank you for everything!
  • Mentors:
    • Stephanie Greufe
    • Reid Hayward
  • Advisor:
    • Nick Horianopoulos
  • FSI Director:
    • Lori Ball
  • Sponsors:
    • Kinder Morgan Foundation
    • Xcel Energy Foundation
    • Frank and Gloria Walsh Science Foundation
    • Suncor Energy