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Biology 22

Biology 22. Drosophila Identification of various phenotypes. Reference for Pictures:. Carolina Drosophila melanogster Manual (45-2620) Published: Carolina Biological Supply Company. The life Cycle. Life Cycle: Drosophila melanogaster. Eggs: contain filaments 2 molting phases “instars”

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Biology 22

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  1. Biology 22 Drosophila Identification of various phenotypes.

  2. Reference for Pictures: • Carolina Drosophila melanogster Manual (45-2620) • Published: Carolina Biological Supply Company

  3. The life Cycle

  4. Life Cycle:Drosophila melanogaster • Eggs: contain filaments • 2 molting phases “instars” • Life cycle is 10 days

  5. Fruity Fly Egg and Larva

  6. Fruit Fly Pupa and Adult

  7. Identification of: • Gender • Body Color

  8. Wild Type Versus Mutants • Body Color • Wild type: Beige • Mutant: Yellow body

  9. Parental phenotypesExperiment: body color • A= female: wt (beige) • B=female: wt (beige) • C=male: wt (beige) • D=female: mutant (yellow) • E=male: mutant (yellow)

  10. Fruit Fly Body Color

  11. Sex combs: Male Drosophila

  12. Abdominal segements • 7 segments to female abdominal segments • 5 segments to male abdominal segments • Male body size is smaller than female • But the “last” abdominal segment is larger in male

  13. Nap TimeTriethylamine

  14. How to anesthetize a fly

  15. How to use the Napper

  16. View the Fruit Fly

  17. You will record class data • After counting the crosses record the data on the class spreadsheet

  18. Experiment 2 3 point test cross Eye color Body color Wing length 3 point test cross: Experiment 2

  19. 1. Body Color 2. Wing length 3. Eye Color ♦Wt vs. Mutant Beige (bl+) vs. Black (bl) Long(vg+) vs. Vestigal(vg) Brick red (br+) vs. brown (br) 3 point test cross for:

  20. Crosses: Female Males • Males and female progeny from this F2 cross are your 3 point test cross results! bl+ vg+ Bw+ bl vg bw X bl vg bw bl vg bw

  21. There are all sorts of eyes!

  22. Eyes

  23. You will have only red eyes (wt)

  24. Fruit Fly Wings

  25. 1866-1945

  26. Parental phenotypes • A= female: wt beige body • B=female: wt beige body • C=male: wt beige body • D=female: yellow body • E=male: yellow body

  27. Female and Male Fruit Flies

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