introduction to dna microarray technology
Download
Skip this Video
Download Presentation
Introduction to DNA Microarray Technology

Loading in 2 Seconds...

play fullscreen
1 / 9

Introduction to DNA Microarray Technology - PowerPoint PPT Presentation


  • 249 Views
  • Uploaded on

Introduction to DNA Microarray Technology. Steen Knudsen, April 2005. DNA Microarrays consist of 100 - 1 million DNA probes attached to a surface of 1 cm by 1 cm (chip). By hybridisation, they can detect DNA or RNA:. If the hybridised DNA or RNA is labelled fluorescently it can

loader
I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
capcha
Download Presentation

PowerPoint Slideshow about 'Introduction to DNA Microarray Technology' - tilly


An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript
slide2
DNA Microarrays consist of 100 - 1 million DNA probes attached to a surface of 1 cm by 1 cm (chip).By hybridisation, they can detect DNA or RNA:

If the hybridised DNA or RNA is labelled fluorescently it can

be quantified by scanning of the chip.

dna microarrays can be manufactured by
DNA microarrays can be manufactured by:
  • Photolitography (Affymetrix, Febit, Nimblegen)
  • Inkjet (Agilent, Canon)
  • Robot spotting (many providers)
affymetrix photolitography
Affymetrix photolitography
  • Each probe 25 bp long
  • 22-40 probes per gene
  • Perfect Match (PM) as well as MisMatch (MM) probes
image analysis
Image Analysis
  • Gridding: identify spots (automatic, semiautomatic, manual)
  • Segmentation: separate spots from background. Fixed circle (B), Adaptive circle C, Adaptive shape (D), Histogram
  • Intensity extraction: mean or median of pixels in spot
  • Background correction: local or global
ad