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Effects of Physiological Folate Concentrations on IC50 Values of 5-MeTHF, FA, and THF

This study investigates the IC50 values of 5-Methyl-Tetrahydrofolate (5-MeTHF), Folic Acid (FA), and Tetrahydrofolate (THF) under physiological conditions. The IC50 values were measured in the presence of various concentrations of folate, revealing significant differences in their inhibitory effects. Notably, the presence of physiological concentrations (0.023 µM) compared to elevated levels (0.23, 2.3, and 23 µM) resulted in increased IC50 factors. This highlights the importance of folate concentration in modulating the efficacy of these compounds.

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Effects of Physiological Folate Concentrations on IC50 Values of 5-MeTHF, FA, and THF

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  1. 0.023 0.023 0.23 0.23 2.3 2.3 23 c 23 5.5 24.9 65.07 4.7 44.9 171.3 1.2 1.5 2.3 1.1 1.1 2.1 Table S1 5-Me-Tetrahydrofolate (5-MeTHF) Folic acid (FA) Tetrahydrofolate (THF) IC50s (nM) in physiological folate (FA 0.023 µM) Increased factor of IC50sa in presence of 0.23, 2.3 and 23 µM FA IC50s (nM) in physiological folate (THF 0.023 µM) IC50s (nM) in physiological folate (5-Me-THF 0.023 µM) Increased factor of IC50a in presence of 0.23, 2.3 and 23 µM of THF Increased factor of IC50sa in presence of 0.23, 2.3 and 23 µM of THF 0.023 0.23 23 2.3 PM b 875.2+109 7.5 21.82 47.44 797+236 1061+400 1.1 1.9 ND 11.8+0.14 4.7 44.9 99.47 9.60+1.8 10.84+9 2.1 2.5 ND CCG b 31.47+17 1.55 1.67 2.1 23.84+1.8 32.12+20 1.43 2.3 ND MTX 59.33+10 1.1 1.18 1.8 67.68+9.8 55.36+11.8 1.83 2.05 ND AMP TMX 14.8+8 1.2 3.3 3.6 3.6+1.8 1 1.8 2.4 19.1+16 0.9 0.8 ND aIncrease factor of IC50s represents the ratio of IC50 in physiological folate condition versus the IC50 in presence of high folate condition. bIncrease value of PM and CCG in bold are all statistically significant (p<0.05) c concentration of 23 uM of 5-Me-THF was inhibitory

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