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In vitro differentiation of T cells from naïve CD4 T cells is achieved by stimulating their T cell receptor (TCR) in the presence of appropriate cytokines. Here we describe a comprehensive protocol for in vitro differentiation of Th0, Th1, Th2, Th17 and iTreg T cells from mouse CD4 T cells.

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in vitro differentiation of t cells

In Vitro Differentiation of T Cells

In vitro differentiation of Th cells from naïve CD4+ T cells is achieved by stimulating

their T cell receptor (TCR) in the presence of appropriate cytokines. Here we describe

a comprehensive protocol for in vitro differentiation of Th0, Th1, Th2, Th17 and iTreg

T cells from mouse CD4+ T cells.

Reagents:

Antibodies: anti-CD28, anti-CD3, anti-IL-4 and anti-IFN-γ antibodies

Cytokines: IL-2, IL-4, IL-6, IL-12, TGF-β

RPMI1640

Sterile PBS

Procedures:

Day 1: Coat wells with anti-CD3 and CD28 antibody

1. Prepare anti-CD3 and anti-CD28 antibodies at 1μg/mL in sterile PBS.

2. Add 250~500 μL total volume per well to 24-well plates.

https://www.creative-diagnostics.com/in-vitro-differentiation-of-t-cells.htm